高效液相色谱法快速检测食品中苏丹红的含量 |
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引用本文: | 曹丽芬,姚黎霞,何良兴,茹巧美.高效液相色谱法快速检测食品中苏丹红的含量[J].安徽农业科学,2014(5):1532-1533,1545. |
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作者姓名: | 曹丽芬 姚黎霞 何良兴 茹巧美 |
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作者单位: | 浙江省杭州市余杭区农产品监测中心; |
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摘 要: | 目的]建立快速检测食品中苏丹红(苏丹红I、Ⅱ、Ⅲ、IV)含量的高效液相色谱法,简化样品的前处理,降低成本,缩短仪器检测时间。方法]选用Athena C18-XDB色谱柱(4.6mm×150.0mm,3.5um),以乙腈水溶液和乙腈丙酮溶液为流动相进行等度洗脱,通过二极管阵列检测器检测食品中苏丹红。结果]该方法检测线性良好,相关系数R≥0.9991,检出限为0.01mg/L,相对标准偏差(n=10)为0.9%~2.0%,回收率为90%~96%。结论]该法具有较高的准确度和精密度,适用于食品中苏丹红含量的测定。
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关 键 词: | 高效液相色谱 苏丹红 食品 |
High Performance Liquid Chromatographic Method for Rapid Determination of Sudan in Food |
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Institution: | CAO Li-fen et al (Agricultural Products Monitoring Center in Yuhang District of Hangzhou City, Hangzhou, Zhejiang 311119) |
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Abstract: | Objective] A analytical method was established to determine Sudan (Sudan I, II, III , IV) content in food by high performance liquid chromatography (HPLC) with simplified sample pre-treatment, reduced cost and shorten instrument detection time. Method] The Sudan were separated on an Athena C,s-XDB column (4.6 mm × 150.0 mm, 3.5 um) by isocratic elution using acetonitrile water solution and acetonitrile acetone solution as mobile pbase, and analyzed with a diode-array detector. Result] The method showed a good linear relationship with correlation coefficient of more than 0. 999 1. The limit of detection (LOD) was 0. 01 mg/L, the relative standard deviation ( n = 10) was between 0.9% -2.0% , and the recovery rate was between 90% -96%. Conclusion] The method has a high accuracy and precision, which is suitable for analyzing the Sudan content in food. |
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Keywords: | High performance liquid chromatography (HPLC) Sudan Food |
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