耐盐红螺菌对水产养殖病害细菌的拮抗作用

为探明光合理细菌对水产养殖动物病害的生物防治功能，对病鳖、鳗及南美白对虾动物体上的病原菌进行分离、纯化与鉴定，人工感染健康动物检测致病性；探讨耐盐红螺菌对病害细菌分离株的仰菌效能，和有效抑菌作用时细胞所处的生长期。试验结果表明，水产动物病害细菌分离株为孤菌属，气单胞菌属和假单胞菌属菌株；耐盐红螺菌的代谢产物对商害细菌分离株均有抑制作用，其平坂柳菌圈为1.2-1.4cm，最低抑菌浓度多为4-8倍稀释液；耐盐红螺菌的拮抗物质随细胞对数生长过程中生成，至细胞衰亡期达到最大值。

The antagonistic action of salt-resistant Rhodospirillaceae bacteria on aquacultural pathogens

The pathogens from ill turtles, eels and Ictiobus cytrinellus were separated, purified and identif ied to prove up the biocontrol function of Photosynthetic bacteria ( PSB ) on diseases of aquatic animals. The pathogenicity of pathogens is examined by artificially infecting the healthy animals. And the antibacterial abilities of sal-t resistant Rhodospirillaceae bacteria against those separated pathogens were studied. At the same time, the phases that PSB produces ef fective antibacterial action were determined. The results show that the separated pathogens from aquatic animals are Vibrio , Aeromonas, and Pseudomonas. The metabolic products of sal-t resistant Rhodospirillaceae bacteria show the bacteriostastic activity to those separated pathogens: the diameter of ant ibacterial zone is 1. 2- 1. 4cm, the minimal inhibitory concentrat ions ( MIC) are mostly at the diluted times 4 - 8. The antagonistic substances of sal-t resistant Rhodospirillaceae bacteria are produced in the exponential phase initially and reached maximal concentration in the contabescence phase.