棉花红腐病菌nit突变体筛选及营养亲和群测定

 以自安徽不同地区分离的棉花红腐病菌（Fusarium moniliforme）单孢株为供试菌株，在含KClO₃培养基上诱导筛选获得抗氯酸盐的硝酸盐营养缺陷型(nit)突变株232株，根据在MM、NM、HM等3种不同氮源培养基上的生长情况划分出nit A、nit B、nit C、nit D 4种突变类型，其中nit A出现频率最高，179株，占总体的77%；nit B 25株，占总体的11%；nit C 24株，占总体的10%；nit D最少，4株，占总体的2%。将各菌株的nit A突变株和测试菌株的nit B或nit C配对，结果12个菌株分为6个不同的营养体亲和群(VCGs)，其中3个VCGs各含1个菌株，另外3个VCGs各含3个菌株。结果还表明，来源于同一野生菌株的不同类型nit突变体间或同一VCG不同野生菌株的nit突变体间可产生互补反应而形成亲和带，其中以nit B型突变株互补性最好，在利用nit突变体测定营养体亲和性时作为标准菌株；来源于不同VCG的nit突变体间不能产生互补反应。

Selection of Nit Mutants and Determination of Vegetative Compatibility Groups in Fusarium moniliforme from Cotton

Cotton red rot caused by Fusarium moniliforme seriously endangers cotton by causing the rot of buds,stem bases and roots of cotton at seedling phase,and resulting in boll rot in boll stage.The incidence and severity of the disease have been developing with the extending of Bt-transgenic cotton in the recent years in Anhui,China.In order to explore the population structure of the causal organism,the vegetative compatibility groups(VCGs) of the pathogenic fungus were tested by applying the nitrate nonutilizin...