牛RPLP1基因的克隆和生物信息学分析

采用RT-PCR法克隆牛RPLP1基因,利用生物信息学分析软件对该基因进行生物信息学分析,应用半定量RT-PCR方法对该基因的组织表达谱进行分析。结果表明,牛RPLP1基因全长为502 bp,包含345 bp的编码区序列,编码114个氨基酸;拓扑预测表明,RPLP1蛋白可能存在3个酪蛋白激酶Ⅱ磷酸化位点,2个豆蔻酰化位点,含有一个Ribosomal-P1保守结构域;牛RPLP1基因在多种组织中均表达,在骨骼肌、肝脏和心肌中表达量较高。

Cloning and Bioinformatics Analysis of the RPLP1 Gene in Cattle

In the present study,the RPLP1 gene was cloned by RT-PCR assay and was subsequently bioinformatically analyzed with bioinformatics analysis software,and the tissue expression profile of this gene was determined by semi-quantitative RT-PCR method.The results showed that cattle RPLP1 gene was composed of 502 nucleotides,including a single open reading frame of345 bp which encoded 114 amino acids;topology prediction analysis showed that there were three casein kinase Ⅱphosphorylation sites,two N-myristoylation sites and one Ribosomal-P1 conserved domain in RPLP1 protein;semi-quantitative RT-PCR assay showed that RPLP1 gene was widely expressed,and high expression was observed in skeletal,liver and cardiac muscle.