散养淮南王土鸡J亚群禽白血病的诊断

对湖北省某农户散养的12周龄淮南王土鸡病鸡进行剖检,发现病鸡肝脏有白色肿瘤;心肌增厚,有疑似肿瘤赘生物;脾脏萎缩,切面呈暗色。用J亚群禽白血病病毒（ALV-J）特异性引物进行PCR检测,扩增出了约545 bp的条带,病鸡组织内含有ALV-J病毒核酸序列,表明该病例为ALV-J感染。

Diagnosis of Subgroup J Avian Leukosis of Scatter-feed Huainanwang Native Chicken

Abstract ：The diseased 12-week-old scatter-feed Huainanwang native chickens in a farm were anatomized. The white tumor was found in liver of the diseased chicken; The myocardium was found thickened and suspected tumor vegetation was found; The splenatrophy was observed and the color of the section was dark. The suspected case was molecularly diagnosed by PCR assay using specific primers of subgroup J avian leukosis virus. A specific DNA band with the size of 545 bp was obtained, indicating that the tissues of the diseased chicken were positive for subgroup J avian leukosis virus. In a conclusion, the suspected ease was pathologically and molecularly diagnosed as infection of subgroup J avian leukosis virus.