松材线虫种群遗传多样性AFLP标记的建立及其应用

 松材线虫是极具危险性的外来入侵生物,其引起的松材线虫病,目前正在我国部分地区迅速扩展和蔓延,对我国林业生产造成了严重的经济损失。开展松材线虫的种群遗传学研究,是了解其成功入侵和爆发成灾内在机理的重要途径。但迄今为止,尚未找到很有效的分子标记方法来检测松材线虫入侵种群的遗传变异。本文采用AFLP分子标记技术,通过对各反应体系和反应条件的优化及对多态性引物组合的筛选,成功地建立了松材线虫的AFLP分子标记实验体系,并筛选出52对高效多态性的引物组合。应用4对引物对27个松材线虫种群样品进行遗传多样性检测,结果表明,AFLP是进行松材线虫种群遗传学研究的一种很灵敏和可靠的分子标记。此外,本文还对AFLP技术在松材线虫研究中的应用前景进行了讨论。

Use of the amplified fragment length polymorphism technique for genetic diversity analysis of the pine wood nematode (Bursaphelenchus xylophilus)

Pine wood nematode(Bursaphelenchus xylophilus) is a dangerous alien species which causes a destructive forest disease-pine wilt disease.This disease is now spreading rapidly and causing serious damages to pine trees in China and some other areas of Asia.In order to understand the intrinsic mechanism of its successful invasion and outbreak,it is necessary to study the genetic variance among native and invasive populations of PWN.But so far,there isn't an available DNA molecular marker applied successfully in monitoring population genetic variance of PWN.This is the first study to apply amplified fragment length polymorphism(AFLP) technique for monitoring the genetic variance of PWN.An AFLP protocol was established that is suitable for PWN population analysis.52 pairs of available primer-combinations were screened,with which rich polymorphic bands were produced that can be used for analysis of genetic diversity ofPWN.By detecting genetic variances of 27 samples of different populations of PWN with four pairs of primers,the result showed that AFLP is a sensitive and reliable molecular marker technique for studying population genetics of PWN.Moreover,the prospects of AFLP application in the study of PWN were also discussed in this paper.