Characterization,specificity and sensibility of produced anti-<Emphasis Type="Italic">Rhamdia quelen</Emphasis> vitellogenin in Brazilian fish species |
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Authors: | Daniele Dietrich Moura Costa Dandie Antunes Bozza Luiz Eduardo Rizzo Juan Garcia Michele Dietrich Moura Costa Ciro Alberto de Oliveira Ribeiro |
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Institution: | 1.Laboratório de Toxicologia Celular, Departamento de Biologia Celular,Universidade Federal do Paraná,Curitiba,Brazil;2.Departamento de Patologia Básica,Universidade Federal do Paraná,Curitiba,Brazil;3.Esta??o de Piscicultura Panamá,Paulo Lopes,Brazil;4.Departamento de Biologia Estrutural, Molecular e Genética,Universidade Estadual de Ponta Grossa,Ponta Grossa,Brazil |
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Abstract: | Endocrine-disrupting chemicals (EDCs) are widespread used and can interfere on hormone regulation with adverse consequences for both biota and human. Vitellogenin (vtg) is a yolk precursor protein synthesized by the liver in response to estrogen. In order to characterize the vtg of tropical fish Rhamdia quelen and establish a molecular biomarker, adult male individuals were exposed to 17-β-estradiol (E2) for vtg induction and anti-R. quelen vtg polyclonal antibodies production. Vitellogenic female fish were used as positive control group. E2-induced vtg was characterized as a glycolipophosphoprotein of high molecular mass with peptide mass fingerprint very similar in E2-exposed male and vitellogenic female fish. A polyclonal serum containing anti-R. quelen vtg antibodies was produced and showed high specificity and sensibility to detect the vtg of three fish species: R. quelen, Piaractus mesopotamicus and Prochilodus lineatus. Wildlife and laboratory studies reported that EDCs released into the environment may alter the levels of plasma vtg in male fish, making this protein a valuable biomarker of xenoestrogens exposure. Then, we propose the use of anti-R. quelen vtg as a tool for biomonitoring studies and water quality assessment in Brazil and South American countries where the three fish species occur. |
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