| 侧孢短芽孢杆菌S62-9产抗菌物质的分离纯化及部分特性的研究 |
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| 引用本文： | 杨倩,于宏伟,郭润芳,贾英民.侧孢短芽孢杆菌S62-9产抗菌物质的分离纯化及部分特性的研究[J].河北农业大学学报,2010,33(2). |
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| 作者姓名： | 杨倩 于宏伟 郭润芳 贾英民 |
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| 作者单位： | 1. 河北农业大学,食品科技学院,河北,保定,071001
2. 河北科技大学,生物科学与工程学院,河北,石家庄,050018 |
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| 基金项目： | 河北省十一五科技计划项目(06220106D-1) |
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| 摘 要： | 为开发新型天然抗菌物质,本研究采用硫酸铵盐析、DEAE-cellulose 52离子交换层析、Sephacryl S-100分子筛层析等步骤对侧孢短芽孢杆菌(Brevibacillus laterosporus)S62-9产的抗菌物质进行分离纯化,并对其部分特性进行研究。结果表明:该抗菌物质对热和pH值稳定性高,且抑菌谱广,可抑制多种革兰氏阳性菌、革兰氏阴性菌和真菌,显示出该抗菌物质具有良好的应用前景。
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| 关 键 词： | 侧孢短芽孢杆菌 抗菌物质 抑菌谱 |
Study on purification and partial properties of antimicrobial substance from Brevibacillus laterosporus S62-9 |
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| YANG Qian,YU Hong-wei,GUO Run-fang,JIA Ying-min.Study on purification and partial properties of antimicrobial substance from Brevibacillus laterosporus S62-9[J].Journal of Agricultural University of Hebei,2010,33(2). |
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| Authors: | YANG Qian YU Hong-wei GUO Run-fang JIA Ying-min |
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| Institution: | 1.College of Food Science and Technology;Agricultural University of Hebei;Baoding 071001;China;2.College of Bioscience and Bioengineering;Hebei University of Science and Technology;Shijiazhuang 050018;China |
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| Abstract: | In order to explore new natural antimicrobial substance,we purified a kind of antimicrobial substance from a strain of Brevibacillus laterosporus S62-9 by using ammonium sulphate precipitation,DEAE-52 column and SephacrylTMS-100 column,etc.Furthermore,the partial properties of the purified substance were studied.The antimicrobial substance was shown thermostable,resistant to pH variation,and a broad spectrum of antagonistic activity against various species of Gram-positive bacteria,Gram-negative bacteria an... |
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| Keywords: | Brevibacillus laterosporus antibacterial substance inhibitory spectrum |
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