| 基于mtDNA ND1基因的家蚕进化分析 |
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| 引用本文： | 赵越,胡小龙,曹广力,薛仁宇,贡成良.基于mtDNA ND1基因的家蚕进化分析[J].江苏蚕业,2009,31(1):5-9. |
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| 作者姓名： | 赵越 胡小龙 曹广力 薛仁宇 贡成良 |
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| 作者单位： | 苏州大学医学部,江苏苏州215123 |
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| 基金项目： | 国家重点基础研究发现计划973计划项目(编号2005CB121000) |
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| 摘 要： | 对中国青州野桑蚕mtDNA中ND1基因进行了克隆和序列分析。结果显示,野桑蚕ND1基因全长为933bp,编码310个氨基酸残基,A+T含量较高,达78.7%;同源性分析显示,不同来源的家蚕和野蚕ND1基因在核苷酸水平和氨基酸水平具有很高的相似性;以ND1基因的核苷酸序列及氨基酸残基序列进行了家蚕及野桑蚕的分子进化分析,进一步证明家蚕起源于中国野桑蚕。用mtDNA的ND1基因的核苷酸序列Blast家蚕的基因组序列,发现家蚕基因组中存在与ND1基因部分区域同源的序列,表明家蚕基因组中存在起源于线粒体的假基因。
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| 关 键 词： | 野桑蚕 mtDNA Nd1基因 假基因 分子进化 |
Phylogenetic Analysis of Bombyx mori based on the ND1 Gene of mtDNA |
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| ZHAO Yue HU Xiaolong CAO GuangLi XUE RenYu GONG ChengLiang.Phylogenetic Analysis of Bombyx mori based on the ND1 Gene of mtDNA[J].Jiangsu Sericulture,2009,31(1):5-9. |
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| Authors: | ZHAO Yue HU Xiaolong CAO GuangLi XUE RenYu GONG ChengLiang |
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| Institution: | Medical College of Soochow University;Suzhou 215123;China |
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| Abstract: | The ND1 gene from Bombyx mandarina strain Qingzhou were cloned using PCR.Sequence result revealed the fragment;933bp encoded 310 amino acids and is rich in A and T,about 78.7%.The alignment analysis of various Bombyx mori L.and Bombyx mandarrina M.shows their ND1 genes are high homologous at nucleotide and amino acid level.Molecular phylogenetic analysis based on the sequence of ND1 gene reflected that Bombyx mori have the closest relatives with Chinese Bombyx mandarina,it indicated that Bombyx mori possibl... |
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| Keywords: | Bombyx mandarina mtDNA ND1gene pseudogene molecular evolution |
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