JEV、PPV、PRRSV、PRV多联PCR的应用研究

用 JEV、PRRSV二联 PCR,PPV、PRV二联 PCR以及这 4种病毒 4联 PCR对来自内蒙、广州、广西、天津、北京、吉林病料进行检测 ,共检了 1 4 6份病料。其中 PRRSV阳性 7份 ,PPV阳性 1 2份 ,PRV阳性 2 1份 ,PRV和 PPV混合感染 4份。并对 5份人工接种 JEV小鼠病料检测 ,其中 4份为阳性。随后对部分阳性 PCR扩增产物进行点杂交和核苷酸测序鉴定 ,证实了 PCR扩增准确性。对内蒙 PRRSV阳性扩增带测序结果显示 ,我国流行 PRRSV为美洲型 ,在扩增片段的核苷酸序列上有 3个碱基差异

Application of JEV,PPV,PRRSV and PRV with Multiple PCR

This experiment tested 146 samples of pathogenic tissues from Inner Mongolia, Guangzhou, Guangxi,Tianjin,Beijing and Jilin with JEV and PRRSV double PCR,PPV and PRV double PCR and the four viruses multiple PCR.Among them,7 were PRRSV positive,12 were PPV positive,21 were PRV positive, 4 were PPV and mixed positive. Five JEV samples of artificially infected mice were tested, 4 were positive.Then some of positive products of PCR were used to make dot hybridization and sequence nucleotide.PCR product was confirmed to be correct.The result of Inner Mongolia in China PRRSV positive product sequencing displayed PRRSV was America type.There were three basic radical differences in nucleotide sequence.