新生仔猪不同阶段睾丸间质细胞增殖数量及其超微结构变化

对１～４周龄仔猪睾丸间质细胞的形态学发育过程进行了光镜和电镜观察。结果：（１）随仔猪年龄增长，间质细胞总数明显增多，３周龄时达到高峰，４周龄时出现下降趋势，间质细胞密度随细胞总数增多而逐渐增加。周围曲细精管逐渐发育成形，至３周龄时与间质组织界限已渐明确；（２）随仔猪日龄的增长，间质细胞内线粒体、滑面内质网、粗面内质网、高尔基复合体和胞浆内膜性分泌小泡的数量显著增多，至３周龄时细胞内出现黑色内噬体。生后３周内，间质细胞核由梭状变为圆形，由小变大，核膜平展，核内质饱满；核仁着色加深，体积增大，显示了较强的基因转录和蛋白质合成的功能。结果表明，仔猪出生后体内雄激素的水平会出现一个新的高峰，其性分化的过程大约需要３周时间。

Observations of Testicular Tissues by Photo and Electron microscopy in Early Stage of Pig Life

The morphological changes in the tissues of testis and the ultrastructure of the Leydig cells of neonatal pigs in early (1 to 4 weeks) stage of life were observed by using photo and electron microscopy. The results indicated that (1) the whole population of Leydig cells increased obviously reaching to the maximum in the third week and then decreased. Seminiferous tubles were taking shape and there were obvious gaps between tubes and interstitial tissues. (2) The number of mitochondria, smooth and rough endoplasmics, Golgi complexes and the various transport vesicles in LC increased continuously from 1 to 3 week of old, the shape of nucleus turned from shuttle like to round and the size of nucleus and nucleolus got larger and more condense in the meantime. Some autophagosomes were observed in 3 week aged Leydig cells. The observations demonstrate that Leydig cells enhance their steroidogenic activity and their protein synthetization during the first 3 weeks. The results suggesed that the active steroidogenics and enzyme synthesization of the Leydig cells during early life stage of neonatal pig would play a major role in the perinatal sexual differentiation.