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| 转基因克隆绵羊的亲子鉴定 | |
| 引用本文： | 代蓉,周平,沈思军,甘尚权,李宁,刘守仁.转基因克隆绵羊的亲子鉴定[J].畜牧与兽医,2010,42(11). |
| 作者姓名： | 代蓉 周平 沈思军 甘尚权 李宁 刘守仁 |
| 作者单位： | 1. 新疆石河子大学,新疆,石河子,832000;新疆农垦科学院,新疆,石河子,832000 2. 新疆农垦科学院,新疆,石河子,832000 3. 新疆石河子大学,新疆,石河子,832000 4. 中国农业大学生物学院,北京,100193 |
| 基金项目： | 新疆生产建设兵团博士资金项目，农业部转基因生物新品种培育重大专项 |
| 摘 要： | 利用IASG上公布的绵/山羊的11个微卫星标记,鉴定转基因克隆羊与供体细胞系和代孕母羊间的亲子关系。结果表明转基因克隆羊与供体细胞系之间11个微卫星标记的基因型一致,而与代孕母羊基因型差异很大,由此确定转基因克隆羊来源于供体细胞系。 |
| 关 键 词： | 转基因克隆 绵羊 微卫星标记 亲子鉴定 |
Parentage identification of sheep from gene transferred somatic cells |
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| DAI Rong,ZHOU Pin,SHEN Si-jun,GAN Shang-quan,LI Ning,LIU Shou-ren.Parentage identification of sheep from gene transferred somatic cells[J].Animal Husbandry & Veterinary Medicine,2010,42(11). | |
| Authors: | DAI Rong ZHOU Pin SHEN Si-jun GAN Shang-quan LI Ning LIU Shou-ren |
| Abstract: | Two transgenic cloned sheep from the same somatic cell line and two pregnant sheep,together with the somatic cell line,were genetically analyzed using 10 microsatellite markers from IASG.According to the data obtained in the current experiment,the RCP for cloned sheep and a donor for cloned somatic cell was 99.999%.The genotypes of cloned sheep were same as the donor cell,but different from the pregnant sheep.In conclusion,two cloned sheep were originally come from the donor. |
| Keywords: | transgenic clone sheep microsatellite loci parentage identification |
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