Identification of a CAPS marker tightly linked to the Tomato yellow leaf curl disease resistance gene <Emphasis Type="Italic">Ty-1</Emphasis> in tomato |
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Authors: | Ana Pérez de Castro José Miguel Blanca María José Díez Fernando Nuez Viñals |
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Institution: | (1) Instituto de Conservación y Mejora de la Agrodiversidad, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain |
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Abstract: | During the process of breeding programmes, several resistance genes have been introgressed into tomato (Solanum lycopersicum) cultivars from different wild tomato relatives. A number of these resistance genes have been mapped to chromosome 6. Among
them, Ty-1 and Mi, which confer resistance to Tomato yellow leaf curl disease and to Meloidogyne spp., respectively, are in most cases incorporated in commercial hybrids. Several molecular markers tightly linked to Mi have been identified. This study was conducted in order to find an informative molecular marker linked to Ty-1. Six markers mapped in the same region as Ty-1 were analysed in plant material carrying different combinations of Ty-1 and Mi alleles. Three of the six markers revealed polymorphism among the assayed accessions. One allele of JB-1 marker showed association
with Ty-1. Furthermore, the presence of Mi did not interfere with the results. The analysis of several accessions of wild tomato relatives with the three polymorphic
markers allowed the establishment of the origin of the alleles found in cultivated plant material, showing that introgressions
from S. lycopersicum, S. pimpinellifolium and S. habrochaites will not interfere with the results of this marker which tags Ty-1. Furthermore this analysis enabled the location of CT21, the RFLP marker from which JB-1 was designed. |
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Keywords: | Marker-assisted selection Mi gene Solanum lycopersicum TYLCD |
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