Distribution of antibody in different immunoglobulin classes in experimental Streptococcus agalactiae infection of the bovine udder |
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| Authors: | E F Logan D P Mackie D J Meneely |
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| Affiliation: | 1. J. C. Bose University of Science and Technology, YMCA, Faridabad, India;2. Central University of Haryana, Mahendergarh, India;3. Central University of Punjab, Bathinda, India;1. Center for Electrochemical Science & Materials, Department of Engineering Chemistry, K.L.E. Institute of Technology, Hubballi 580027, Karnataka, India;2. Electrochemical Research Group, Department of Chemistry, St. Joseph''s College (Autonomous), Lalbagh Road, Bangalore 560027, Karnataka, India;3. Pharmaceutical Engineering, Sonia College of Pharmacy, Dharwad 580 002, Karnataka, India;1. Department of Pediatric Infectious Diseases, University of Washington, Seattle, Wash;8. Department of Global Health, University of Washington, Seattle, Wash;2. Seattle Children''s Research Institute, Seattle, Wash;3. Department of Biology and Center for Microbial Sciences, San Diego State University, San Diego, Calif;4. Department of Pediatrics, University of California San Diego School of Medicine, La Jolla, Calif;5. Department of Biomedical Sciences and Veterinary Public Health, Swedish University for Agricultural Sciences, Uppsala, Sweden;6. Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden;7. Department of Anatomy, Physiology and Biochemistry, the Swedish University of Agricultural Sciences, Uppsala, Sweden |
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| Abstract: | Ten first lactation heifers, four of which were systemically hyperimmune to Streptococcus agalactiae were infected experimentally via the teat canal into the right front quarter on two or three consecutive occasions at 23- and 30-day intervals. The humoral immune response in the udder and sera was monitored by examining the immunoglobulin class of antibodies present using an enzyme-linked immunosorbent assay (ELISA). In the infected quarter the response was biphasic and at least two different immunoglobulin classes of antibody were involved. In the first phase the antibody was predominantly of the IgA class and reached a peak 24 h after infection and then gradually declined to pre-infection levels. At approximately 72 h IgG antibody appeared in the infected quarter and reached a maximum six days post-infection after which the levels declined slowly. In the control quarters the first phase was absent and IgA did not appear in the quarters. However, after 72 h IgG antibody was present in these quarters. |
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