水稻耐冷性研究Ⅲ.特定颖花结实率作为耐冷性指标的分子依据

以云南稻种耐冷性资源昆明小白谷及弱耐冷性的日本品种十和田配制的杂交F2代为材料,采用159个RFLP及SSR分子标记构建的连锁图和STATISTIC等分析软件,以单株结实率作为耐冷性评价指标.分析结果显示单株结实率与单株特定颖花结实率之间的相关系数(r)为0.8364;与耐冷性(以单株结实率为指标)相关的分子标记有43个,分布在8条染色体

Studies on Cold Tolerance of Rice, Oryza sativa L.Ⅲ.Molecular Basis for Special Fertility Percentage as Evaluation Increterior of Cold Toleance

Here is the third part of a series papers to describe the studies on cold tolerance of rice. This part describes the results obtained by using the parents, Kunmingxiaobaigu, on indigenous variety with cold-tolerance from Yunnan, and Towada, a cold-sensitive variety bred in Japan, as well as their F 2 progenies as tested materials, combining by using 159 RFLP and SSR markers and STATISTIC software. The total fertility percentage (TFP) was used as indicator of cold tolerance. The coefficient of relationship between TFP and special fertility percentage(SFP) of single plant was 0.8364. 43 markers were related to cold tolerance based on the TFP as its indicator, distributed in eleven regions on eight chromosomes while 34 ones related to SFP over nine regions on seven chromosomes. The seven chromosomes related to SFP belonged to the eight ones related to TFP. The nine regions related to SFP belonged to the eleven regions related to TFP. The above results showed that SFP can be used to represent TFP in evaluation of cold tolerance. The issue over SFP to represent TFP in other fields was discussed.