鸡传染性支气管炎病毒核衣壳蛋白基因的克隆、表达及应用

核衣壳蛋白(N)是鸡传染性支气管炎病毒(IBV)的重要结构蛋白。根据已报道的IBV基因序列扩增IBVN基因,割胶回收扩增产物并将其克隆到pET-28a( )载体中,转化大肠杆菌BL21(DE3),经IPTG诱导,目的蛋白得到高效表达。分离纯化重组N蛋白并用其作为抗原建立ELISA检测抗体方法,研究了IBVM41攻毒后雏鸡血清特异性抗体消涨规律。

Cloning, Expression and Application of Nucleocapsid Protein Gene of Avian Infectious Bronchitis Virus

Nucleocapsid protein (N protein) is one of the major structural protein of infectious bronchitis virus (IBV). The N gene fragment of the IBV was amplified based on the reported gene sequences, after purification and withdraw, the products were cloned into pET-28a ( ) vector and transformed into E.coll (DE3), the target protein was successfully expressed. The affinity-purified expressed protein was used as a coating antigen for developing an ELISA for serum detection of the chicken infected by IBV M41. The results showed that the IBV N gene fragment was correctly cloned and expressed, and the target protein could be used to detect the antibody against the N protein of the IBV. The Kinetics of antibody in avian infected by IBV M41 was researched with the recombinant N protein