霍山石斛类原球茎诱导及其发育过程研究

 以霍山石斛试管苗带节茎段为材料, 诱导产生类原球茎, 并研究类原球茎发育过程。结果表明, 霍山石斛类原球茎诱导的适宜培养基为Knudson + 2, 4-D 0.1 mg·L ^{- 1} + KT 0.05 mg·L ^{- 1} , 诱导率为25.50%。以固体静置、固液双层静置、液体静置、液体振荡4种不同培养方式进行增殖培养, 其中液体振荡培养方式更适于类原球茎快速增殖。类原球茎通过胚状体发生途径形成, 类似于合子胚的发育, 即经历原胚、心形胚、鱼雷形胚和子叶胚, 最后发育为成熟胚。类原球茎发育进程大致可分为类原球茎形成期、类原球茎肥大期、茎叶分化期、茎叶形成期以及茎叶伸长期等阶段。

Induction of Protocorm-like Bodies and Its Development from Stem Segments of Dendrobium huoshanense

Stem segments of Dend robium huoshanese were used to induce p rotocorm-like bodies ( PLBs) and the development of PLB s was studied. The results showed that the most suitable medium for PLBs inducing is Knudson + 2, 4-D 0.1 mg·L ^{- 1} + KT 0.05 mg·L ^{- 1} , the induction ratio can reach 25.50% onthismedium. Culture methods also affect the proliferation of PLB s, shaking-bottle liquid cultivation is more suitable for PLB s proliferation among the four culture methods, such as solid culture, double layer, static liquid and haking-bottle liquid. Anatomical investigation revealed that the formation of PLB s from Dendrobium huoshanese is similar to zygotic embryo, development from proem-bryo, globularem-byro, heart-shaped embryo,torpedo-embryo, then from cotyledon to mature embryoid. The development of PLBs can be divided into five stages: Formation of PLBs, hypertrophy of PLB s, differentiation of stalks and leaves, formation of stalks and leaves, stretch of stalks and leaves.