| 秤锤树叶片内生真菌的分离鉴定及其对植株生长的影响 |
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| 引用本文： | 李霞,曹昆,丛伟.秤锤树叶片内生真菌的分离鉴定及其对植株生长的影响[J].广西农业生物科学,2010(1):75-81. |
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| 作者姓名： | 李霞 曹昆 丛伟 |
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| 作者单位： | 江苏省农业科学院粮食作物研究所;江苏省优质水稻工程技术研究中心; |
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| 基金项目： | 国家自然科学基金项目(30871459);;转基因生物新品种培育科技重大专项(2008ZX08001-004)共同资助 |
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| 摘 要： | 本研究从秤锤树叶片中分离内生菌进行分子鉴定,并接种到无菌组培苗中研究其对植株生长的影响。研究结果显示,从秤锤树叶片中共分离获得7株真菌。扩增出的6个菌株经ITS测序,在NCBI网站上进行基因序列比对,鉴定出其中5株菌株均属于球毛壳菌（Chaetomium globosum）,1株属于子囊菌（As-comycete）。进一步实验表明：分离的菌株多数对组培苗的株高生长影响不大,可初步鉴定其为内生菌,其中的属于球毛壳菌的F和H菌株对无菌苗的株高有明显的促进作用。
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| 关 键 词： | 秤锤树 内生菌 转录内间隔区 植物组织培养 |
Isolation and Identification of Endophyte from Leaves of Sinojackia xylocarpa and Their Effect on Plant Growth |
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| Li Xia Cao Kun Cong Wei Institute of Foods Crop,Jiangsu Academy of Agriculture Sciences,Jiangsu High Quality Rice R&D Center,Nanjing.Isolation and Identification of Endophyte from Leaves of Sinojackia xylocarpa and Their Effect on Plant Growth[J].Journal of Guangxi Agricultural and Biological Science,2010(1):75-81. |
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| Authors: | Li Xia Cao Kun Cong Wei Institute of Foods Crop Jiangsu Academy of Agriculture Sciences Jiangsu High Quality Rice R&D Center Nanjing |
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| Institution: | Li Xia Cao Kun Cong Wei Institute of Foods Crop,Jiangsu Academy of Agriculture Sciences,Jiangsu High Quality Rice R&D Center,Nanjing,210014 |
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| Abstract: | Molecular identification of endophytes which isolated from leaves of Sinojackia xylocarpa were completed,and the tissue culture seedlings of Sinojackia xylocarpa were inoculated with these endophytes in order to study the effects of them on the growth of tissue culture seedlings. The results showed that seven strains were isolated totally from leaves of Sinojackia xylocarpa,which all belonged to fungal. Six strains were amplified and sequenced,comparison analyses on the sequences of them from NCBI showed th... |
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| Keywords: | Sinojackia xylocarpa Endophytes Internal transcribed spacer （ITS） Plant tissue culture |
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