利用转座子显示技术鉴别柑橘芽变品种

在依据柑橘全基因组信息的基础上,针对转座子序列开发了一种适合鉴别柑橘芽变品种的分子技术。以43个柑橘芽变品种和7个普通柑橘品种为材料,采用转座子显示技术(transposon display,TD)对转座子进行多态性分析。结果表明:5个DNA转座子(DTM63、DTM58、Helitron1、Spem和mPing2)和4个反转录转座子(Line1、LTR-Gypsy3、LTR-Gypsy5和Tcs2)可以较好地鉴别柑橘不同芽变品种。罗伯逊脐橙及其早熟芽变品种罗脐35号能被DTM63、Spem和mPing2鉴别;m Ping2能鉴别罗伯逊脐橙/罗脐35号、暗柳/红暗柳、普通椪柑/黔阳无核椪柑,以及红肉琯溪蜜柚/橙皮琯溪蜜柚这4对芽变品种。DNA转座子DTH3在甜橙芽变品种中多态性高,可以鉴别不同的甜橙芽变品种。因此,TD技术是一种值得关注的能有效鉴别柑橘芽变品种的分子标记技术。

Identification the Citrus Bud Mutants by Transposon Display Technology

Based on the citrus genome information,this study developed a molecular technology based on the transposon sequences to distinguish bud mutants in citrus.Transposon display (TD) technology was used to investigate the transposon polymorphisms of 43 citrus bud mutant cultivars and 7 wild type citrus cultivars.The results showed that 5 DNA transposons (DTM63,DTM58,Helitronl,Spem and mPing2) and 4 retrotransposons (Linel,LTR-Gypsy3,LTR-Gypsy5 and Tcs2) could clearly identify the differences between bud mutants.Robertson navel orange and its early-maturing cultivar Robertson 35 could be identified by DTM63,Spem and mPing2;mPing2 could identify 4 pairs of bud mutants as following:Robertson navel orange and Robertson 35,Anliu and Hong Anliu,the common ponkan and Qianyang seedless ponkan,red flesh and orange skin of Guanxi pummelo.DTH3,a DNA transposon,was highly polymorphic among sweet oranges,and it could identify different bud mutants in sweet orange.Therefore,TD is a type of noticeable and effective molecular marker technology in the identification of citrus bud mutants.