Diagnosis of botulism types C and D in cattle by a monoclonal antibody-based sandwich ELISA |
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| Authors: | Brooks C E Clarke H J Graham D A Ball H J |
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| Institution: | Veterinary Sciences Division, Agri-Food and Biosciences Institute Northern Ireland, Stormont, Belfast. cathy.brooks@afbini.gov.uk |
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| Abstract: | This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs. |
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