毛细管电泳分析茶黄素的方法研究

研究了毛细管电泳分析茶黄素类组分的最佳条件。结果表明:在250mmol/LH3BO4、10mmol/LKH2PO4、0.75mmol/LSDS和20%(v/v)乙腈组成的pH7.5电泳介质中,选择25kV电压、20℃柱温和200nm波长检测,可在10min以内将茶叶中的四种主要茶黄素单体分离,并获得较高的检测灵敏度。四种茶黄素单体浓度与峰面积值之间的相关系数r=0.9945~0.9990,检测下限在0.53~0.64μg/ml之间,加标回收率为92.50%~108.36%,变异系数≤2.67%。本方法快速、准确,可用于实际样品的测定。

Analysis of Theaflavins by Capillary Electrophoresis

The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250 mmol/L H3BO4, 10 mmol/L KH2PO4, 0.75 mmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25 kV, column temperature 20℃, and detecting wavelength 200 nm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64 μg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.