| 反义ACC氧化酶基因的导入对番茄乙烯生成的抑制作用 |
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| 引用本文： | 叶志彪,李汉霞.反义ACC氧化酶基因的导入对番茄乙烯生成的抑制作用[J].华中农业大学学报,1996,15(4):305-309. |
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| 作者姓名： | 叶志彪 李汉霞 |
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| 作者单位： | 武汉华中农业大学园艺系，武汉华中农业大学作物遗传改良国家重点实验室 |
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| 基金项目： | 国家自然科学基金,湖北省自然科学基金 |
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| 摘 要： | 将反向克隆的ＡＣＣ氧化酶基因通过Ｔｉ质粒导入到番茄基因组中，转基因番茄植株叶片和果实中ＡＣＣ氧化酶活性和乙烯产生速率均受到显著抑制，显示出反义基因能抑制靶基因（ＡＣＣ氧化酶）的表达。子代抗性标记基因的分离结果表明呈单基因遗传。
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| 关 键 词： | 番茄 反义基因 基因表达 ACC 氧化酶 |
INHIBITATION OF INTRODUCING ANTISENSE ACC OXIDASE GENE INTO TOMATO GENOME ON EXPRESSION OF ITS ENDOGENOUS GENE |
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| Ye Zhibiao,Li Hanxi,Zheng Yonglian,Liu Houli.INHIBITATION OF INTRODUCING ANTISENSE ACC OXIDASE GENE INTO TOMATO GENOME ON EXPRESSION OF ITS ENDOGENOUS GENE[J].Journal of Huazhong Agricultural University,1996,15(4):305-309. |
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| Authors: | Ye Zhibiao Li Hanxi Zheng Yonglian Liu Houli |
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| Abstract: | The antisense cDNA clone of 1-aminocycloprapane-1-carboxylic acid (ACC)oxidase gene was ransformed into tomato genome mediated with Ti plasmid vector. The ACC oxidase activity and ethylene production rate were significantly inhibited in the leaves and fruits of transgenic plants. This shows that the expression of the endogenous ACC oxidase gene was down regulated.The inheritance of selective marker gene transferred is in the model of the simple Mendel's fashion in progenies of the self-crossed generation. |
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| Keywords: | tomato antisense gene gene experession ACC oxidase |
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