食用甜蕨及苦蕨黄酮类化合物对DPPH自由基的清除效果

为了弄清食用甜蕨和苦蕨黄酮提取物的抗氧化特性,以VE为对照,采用分光光度法等方法,研究了食用甜蕨和苦蕨黄酮类化合物对DPPH自由基清除率的影响。结果表明:在510nm下,甜蕨和苦蕨黄酮提取液的含量分别为843.59μg/g和614.15μg/g;甜蕨和苦蕨黄酮提取物对DPPH均有较好的清除效果,苦蕨与甜蕨黄酮提取物浓度为12.283μg/mL时,对DPPH的清除率分别为87.13%和50.21%,苦蕨黄酮提取液的抗氧化能力显著高于甜蕨,但二者的抗氧化能力均显著高于VE。

Effects of Flavonoids Extracted from Sweet Fern and Bitter Fern on DPPH Elimination

In order to ascertain the antioxidant properties of flavonoids extracted from sweet fern and bitter fern,the elimination rate on DPPH radical was studied taking VE as the control using spectrophotometric and other methods.The results showed that at 510 nm,the content of flavonoids extract of sweet fern and bitter fern was 843.59 μg/g and 614.15 μg/g respectively.The extraction of the flavonoids from sweet fern and bitter fern on DPPH elimination had The better effect.The flavonoids extracted from the sweet fern and bitter fern on DPPH elimination rate was 50.21% and 87.13% separately when the flavonoids concentration was 12.283 μg/mL.The antioxidant capacity of the bitter fern flavonoids was significantly higher than that of the sweet fern flavonoids,but the antioxidant capacity of the sweet fern and bitter fern were significantly higher than VE.