| 外源钾通道基因在水稻中的表达及其钾吸收特征研究 |
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| 引用本文： | 施卫明,王校常,严蔚东,汤利,安志装,何锶洁,田文忠,曹志洪.外源钾通道基因在水稻中的表达及其钾吸收特征研究[J].作物学报,2002,28(3):374-378. |
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| 作者姓名： | 施卫明 王校常 严蔚东 汤利 安志装 何锶洁 田文忠 曹志洪 |
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| 作者单位： | 中国科学院南京土壤研究所,江苏南京,210008 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9770 43 7，3 970 0 0 83 )，国家重点基础研究规划项目 (G19990 1180 8)，中科院重点项目 (KZ95 2 -
S1-2 2 1)，江苏省自然科学基金 (BK99196)，中科院土壤圈物质循环开放实验室资助。 |
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| 摘 要： | 通过基因枪导入法,将外源钾通道基因导入水稻中花8号、中花9号、中花13以及8706中表达,获得了转基因植株.经PCR和Southern检测,共获得42个表达了钾通道基因的转基因株系.通过二代的筛选并获得了部分株系的纯合系.转基因植株对钾的吸收累积试验表明,与对照相比,供试转基因株系的吸钾速率和对钾的累积能力都有明显提高.研究表明
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| 关 键 词： | 钾通道基因 水稻 基因枪导入法 钾吸收 |
| 收稿时间： | 2001-06-18 |
| 修稿时间： | 2001年6月18日 |
Over-expressing of Potassium Channel Genes in Rice Plants and Its Effect on K Uptake and Accumulation |
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| SHI Wei Ming,WANG Xiao Chang,YAN Wei Dong,TANG Li,AN Zhi Zhuang,HE Si Jie,TIAN Wen Zhong,CAO Zhi Hong.Over-expressing of Potassium Channel Genes in Rice Plants and Its Effect on K Uptake and Accumulation[J].Acta Agronomica Sinica,2002,28(3):374-378. |
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| Authors: | SHI Wei Ming WANG Xiao Chang YAN Wei Dong TANG Li AN Zhi Zhuang HE Si Jie TIAN Wen Zhong CAO Zhi Hong |
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| Institution: | SHI Wei Ming 1 WANG Xiao Chang 1 YAN Wei Dong 1 TANG Li 1 AN Zhi Zhuang 1 HE Si Jie 2 TIAN Wen Zhong 2 CAO Zhi Hong 1 |
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| Abstract: | Potassium channel genes( AKT 1 or KAT 1) were introduced into the rice (Zhonghua 8, Zhonghua 9, Zhonghua 13 and 8706) by biolistic gun. Total 42 transgenic plants been obtained based on the results of PCR and Southern analysis. Some pure transgenic lines were also gotten. The experiments of hydroponic culture illustrated that the transgenic plants have higher capacity of potassium uptake and accumulation. These results showed that over expressing of K + channel genes in plants could improve K nutrition characteristic of crops. |
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| Keywords: | Potassium channel gene Rice Biolistic gun K uptake |
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