Evaluation of the PATHFAST Chemiluminescent Enzyme Immunoassay for Measuring
Progesterone in Whole Blood and Serum of Mares |
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Authors: | Yuko TOISHI Nobuo TSUNODA Masaaki TAGAMI Hiromitsu HASHIMOTO Fumiki KATO Tsukasa SUZUKI Kentaro NAGAOKA Gen Watanabe Shota TOKUYAMA Kiyoshi OKUDA Kazuyoshi TAYA |
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Institution: | 1. Shadai Corporation, Hokkaido 059-1432, Japan;2. Laboratory of Veterinary Physiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan;3. Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan;4. Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan |
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Abstract: | Evaluation of a new chemiluminescent enzyme immunoassay, the PATHFAST assay system
(PATHFAST), for measurement of circulating progesterone in mares was performed. Five mares
at the mid-luteal stage were administrated a single i.m. injection of prostaglandin F2α
analog (PGF2α; cloprostenol 250 μg/ml), and then blood samples were collected from the
jugular vein at 0, 15, 30 and 45 min, at one-hour intervals until 24 and at 48 hr via a
catheter in the jugular vein. To monitor the physiological changes in circulating
progesterone in mares after induced luteolysis, concentrations of progesterone in whole
blood and serum samples were measured by PATHFAST. In addition, concentrations of
progesterone in serum samples measured by PATHFAST were compared with those measured by
radioimmunoassay (RIA) and enzyme immunoassay (EIA). Using PATHFAST, the serum
concentrations of progesterone in mares correlated highly with those of whole blood
samples (r=0.9672, n=88). The serum concentrations of progesterone as measured by PATHFAST
correlated well with RIA (r=0.9654, n=88) and EIA (r=0.9323, n=112). An abrupt decline in
circulating progesterone in whole blood samples was observed within 2 hr (50%), followed
by a gradual decline until 48 hr later. The results for progesterone in whole blood
samples correlated highly with those in serum samples, and the declining pattern
paralleled that of the serum samples. These results demonstrated that PATHFAST is useful
in the equine clinic as an accurate diagnostic tool for rapid assay of progesterone within
26 min, using unextracted whole blood. |
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Keywords: | enzyme immunoassay horses PATHFAST PATHFAST progesterone radioimmunoassay |
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