生防多粘类芽胞杆菌P1悬浮剂的研制

本研究通过流点法、生物相容性、正交试验等方法，对多粘类芽胞杆菌Paenibacillus polymyxa P1悬浮剂（P1-SC）的配方所添加的助剂进行了筛选。结果表明，当P1-SC的配方为：P1菌粉为10%（≥10.18 lg cfu/g）、润湿分散剂500^#和MOA-7均为2.5%、增稠剂硅酸镁铝和黄原胶分别为0.75%和0.15%、防腐剂万立净C25 0.20%、防冻剂丙三醇3%、消泡剂0.3%、并用软水补足至100%时，所制得的P1-SC中的活菌数≥9.18 lg cfu/mL、细度（通过75μm筛）≥95%、pH在5.0～8.0、持久起泡量（1 min后）≤30 mL、倾倒后残余物质量≤5.0%、洗涤后残余物质量≤0.5%、悬浮率≥90%，达到了企业对悬浮剂的检测标准。同时，对P1-SC中P1芽胞在不同温度条件下的货架期进行了测定。6个月的贮存测试结果表明，45℃活菌数下降了6%，而在4℃和25℃ P1的活菌数没有发生明显变化。

Development of Paenibacillus polymyxa P1 Suspension Concentrate

By using the methods of flow point, biocompatibility assay, orthogonal test etc, a variety of screening tests were conducted for all of the adjuvants added in the formulation of suspension concentrate with a strain P1 of Paenibacillus polymyxa (P1-SC). The results showed that, when the formulation of P1-SC was prepared as:P1 was 10% (≥ 10.18 lg cfu/g), both wetting dispersant of 500^# and MOA-7 were 2.5%, thickeners of magnesium aluminum silicate and xanthan gum were 0.75% and 0.15% respectively, antiseptic agent C25 was 0.20%, antifreeze of propanol was 3%, silicone anti-foaming agent was 0.3%, and finally adding soft water to make it up to 100%, the viable P1 spores in P1-SC were ≥ 9.18 lgcfu/mL, the fineness (through 75 µm sieve) was ≥ 95%, the pH value was 5.0-8.0, the amount of long-lasting foaming (after 1 min) was ≤ 30 mL, the residual mass after dumping was ≤ 5.0%, and the residual mass after washing was ≤ 0.5%, the suspension rate was ≥ 90%, which means it reaches the detection standards of an enterprise for a SC formulation. Meanwhile, the shelf life of P1 spores in prepared P1-SC was measured under different temperatures. A 6-month storage test revealed that the number of viable P1 decreased about 6% when stored at 45℃,however, it did not change much when stored at 4℃ and 25℃, respectively.