稻瘟病菌中小G蛋白Rho3的假定互作蛋白MoKin1的功能分析

小G蛋白MoRho3在稻瘟病菌生命活动中具有重要的生物学功能。前期研究表明,MoRho3的缺失对于稻瘟病菌分生孢子的形态建设、附着胞萌发和侵入能力以及对宿主的致病性等表型都存在严重缺陷。为了进一步解析MoRho3在稻瘟病菌中的网络调控途径,通过生物信息学的方法预测了MoRho3的互作蛋白。在众多假定互作蛋白中,蛋白激酶MoKin1与酿酒酵母中KIN1和KIN2是同源蛋白。在酵母细胞中,KIN1和KIN2可以抑制△rho3的表型缺陷。为了验证在稻瘟病菌中MoKin1与MoRho3之间是否存在着相似的调控途径,本研究通过分析MoKin1与同源蛋白的进化关系,序列结构,定位状态以及相关的互作蛋白,揭示了MoKin1的功能特点,为后续病原菌中MoKin1与MoRho3互作机制的研究,以及病原菌致病机制的解析提供了新的思路和方向。

Functional Analysis of MoKin1 as Putative Substrate of the Small GTPase MoRho3 in Rice Blast Fungus,Magnaporthe oryzae

Small GTPase MoRho3 plays an important biological role in the rice blast fungus, Magnaporthe oryzae. The preamble research elucidated that the deletion of MoRho3 in this fungus caused serve phenotypic defects, such as abnormal morphology of conidia, reduction of germination and penetration of appressoria and reduced pathogenicity. In order to understand the mechanism of the regulatory pathways of MoRho3, we adopted the bioinformatics to search the putative interacting protein of MoRho3. We found the homologus protein of Saccharomyces cerevisiae KIN1 and KIN2 in the proteins, named MoKin1. Because the over-expression of KIN1 and KIN2 could rescue some phenotypic defects of △rho3 in S. cerevisiae, in order to verify whether there was a similar regulatory pathway between Mokin1 and MoRho3 in M. oryzae, the study revealed the functional characteristics of Mokin1 by analyzing the evolutionary relationship, sequence structure, localization status and related interaction proteins, which would provide a new basis for the further study of the interaction between Mokin1 and MoRho3 and the pathogenic mechanism of M. oryzae.