珠芽魔芋组培快繁技术

为探索出一套适于珠芽魔芋的组培快繁技术体系,本研究以珠芽黄魔芋不同发育时期的叶片为外植体,开展组培快繁技术研究。结果表明：以开始伸出鳞片叶片作为外植体材料,75%酒精消毒30 s后,0.1% HgCl₂溶液中浸泡15 min为宜,其成活率达到96.7%;最优愈伤组织诱导培养基配方为MS+TDZ 0.5 mg/L+NAA 0.1 mg/L+蔗糖30.0 g/L+琼脂6.0 g/L,其诱导率达到95.6%;最优不定芽诱导培养基配方为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+蔗糖30.0 g/L+琼脂6.0 g/L,不定芽诱导率达到71.1%,单位接种质量愈伤组织分化芽数达到2.88;全展叶片苗在MS+NAA 0.25 mg/L+蔗糖15.0 g/L+琼脂 6.0 g/L培养基的生根率达到100.0%,平均根数达到1.36。以开始伸出鳞片叶片为外植体建立的珠芽黄魔芋组培快繁技术体系,达到了魔芋种苗的规模化生产技术水平,对解决珠芽魔芋产业发展中种芋供不应求的问题具有积极意义。

Tissue Culture and Plant Regeneration of Amorphophallus bulbifer

In order to explore the tissue culture and rapid propagation of Amorphophallus bulbifer, the leaves of different growth period of A. bulbifer were used as explants.The best surface sterilization was achieved by immersing the explants in the solution of 75% alcohol for 30 seconds then in 0.1% HgCl₂ solution for 15 minutes and the survival rate reached 96.7%. The best callus induction medium was MS+TDZ 0.5 mg/L+NAA 0.1 mg/L+sugar 30.0 g/L+agar 6.0 g/L and the callus induction rate reached 95.6%. The best bud induction medium was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+sugar 30.0 g/L+ agar 6.0 g/L on which the bud induction rate and number of differentiation buds of callus with unit inoculation quality reached 71.1% and 2.88. The best medium for rooting was MS+NAA 0.25 mg/L+sugar 15.0 g/L+agar 6.0 g/L, on which the rooting rate reached 100.0% and the average root number reached 1.36. Tissue culture and rapid propagation technology system of A. bulbifer was established by extruding scale leaves as explants,which reached the scale production technology level of konjac seedlings and is of positive significance to solve the problem of shortage of seed taro in the development of konjac industry.