| 不结球白菜S位点受体激酶基因片段的克隆与表达分析 |
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| 引用本文： | 张爱芬,王立,侯喜林,刘同坤,李英.不结球白菜S位点受体激酶基因片段的克隆与表达分析[J].南京农业大学学报,2011,34(3). |
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| 作者姓名： | 张爱芬 王立 侯喜林 刘同坤 李英 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室/农业部南方蔬菜遗传改良重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家973计划项目子课题(2009CB119001-04); 教育部新世纪优秀人才支持计划项目(NCET-07-0439) |
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| 摘 要： | 以不结球白菜自交不亲和系03的基因组DNA和柱头cDNA为模板,利用引物SRKF/SRKR扩增获得长度为964 bp和646 bp的SRK基因片段。序列比较分析表明,克隆的基因片段属于SRK基因的激酶域,该序列包含4个外显子和3个内含子,编码215个氨基酸,与芜菁、甘蓝、芥蓝等SRK基因有90%以上的相似性。荧光定量PCR分析结果表明:自交不亲和系03和自交亲和系105不同组织中SRK基因的表达水平存在显著的差异,SRK基因主要在自交不亲和系的柱头中高度表达,自交亲和系中该基因的表达主要分布于叶片、花蕾和柱头组织中。
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| 关 键 词： | 不结球白菜 S位点受体激酶基因 荧光定量PCR 基因表达分析 |
Cloning and expression analysis of SRK gene fragment in non-heading Chinese cabbage |
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| ZHANG Ai-fen,WANG Li,HOU Xi-lin,LIU Tong-kun,LI Ying.Cloning and expression analysis of SRK gene fragment in non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2011,34(3). |
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| Authors: | ZHANG Ai-fen WANG Li HOU Xi-lin LIU Tong-kun LI Ying |
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| Institution: | ZHANG Ai-fen,WANG Li,HOU Xi-lin,LIU Tong-kun,LI Ying(State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Using primers SRKF/SRKR,964 bp gDNA(BcSRK-1)and 646 bp cDNA sequences of S locus receptor kinase gene were obtained from self-incompatible line 03 of Brassica campestris ssp.chinensis Makino.Sequence alignment and phylogenetic analysis revealed that DNA and cDNA of BcSRK-1 belonged to kinase domain,containing 4 exons and 3 introns,and coded 215 amino acids.Furthermore,its similarity was over 90% with the SRK gene of Brassica rapa,Brassica oleracea and Brassica oleracea var.alboglabra.Real-time PCR analysis ... |
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| Keywords: | Brassica campestris ssp chinensis Makino S locus receptor kinase gene real-time PCR quantitative analysis of gene expression |
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