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| Identification of differentially expressed genes associated with bud dormancy release in tree peony （Paeonia suffruticosa） by suppression subtractive hybridization | |
| 摘 要： | |
| 关 键 词： | 基因克隆 休眠 芍药 杂交化 |
Identification of differentially expressed genes associated with bud dormancy release in tree peony(Paeonia suffruticosa) by suppression subtractive hybridization |
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| Authors: | HUANG Xin ZHENG Guo-sheng DAI Si-lan GAI Shu-peng |
| Institution: | 1. College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P. R. China;College of Life Science, Qingdao Agricultural University, Qingdao 266109, P. R. China 2. College of Life Science, Qingdao Agricultural University, Qingdao 266109, P. R. China 3. College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P. R. China |
| Abstract: | A subtractive cDNA library was developed to study genes associated with bud dormancy release in tree peonies.In order to identify genes that are highly expressed in buds released from dormancy,588 clones were examined by differential screening.Of these,185 clones were selected to be sequenced.A total of 37 unique sequences were obtained of which only 31 sequences have matches in the NCBI database or the Arabidopsis thaliana protein database.Semi-quantitative RT-PCR was used to confirm further the expression... |
| Keywords: | cDNA clone dormancy subtractive hybridization tree peony(Paeonia suffruticosa) |
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