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酸性染料比色法测定断肠草总生物碱的含量
引用本文:陈志慧,宋光泉,周家容.酸性染料比色法测定断肠草总生物碱的含量[J].江西农业大学学报,2006,28(3):440-443.
作者姓名:陈志慧  宋光泉  周家容
作者单位:仲恺农业技术学院,化学与化工系,广东,广州,510225
基金项目:仲恺农业技术学院校科研和教改项目
摘    要:建立了断肠草总生物碱含量的酸性染料比色测定法,并考察了各种因素对测定结果的影响。以溴甲酚绿作为测试用酸性染料,在总生物碱与其形成稳定络合物时间范围内,利用比色法测定了断肠草总生物碱的含量。此方法快速简便、准确易行。

关 键 词:断肠草  总生物碱  酸性染料比色法
文章编号:1000-2286(2006)03-0440-04
收稿时间:2005-12-07
修稿时间:2006-03-23

Determination of Total Alkaloid of Gelsemium elegans Benth by Bromophenol Blue Colorimetry
CHEN Zhi-hui,SONG Guang-quan,ZHOU Jia-rong.Determination of Total Alkaloid of Gelsemium elegans Benth by Bromophenol Blue Colorimetry[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(3):440-443.
Authors:CHEN Zhi-hui  SONG Guang-quan  ZHOU Jia-rong
Institution:Department of Chemistry and Chemical Engineering, Zhongkai College of Agriculture and Technology, Guangzhou 510225, China
Abstract:An acid-dye colorimetry method was developed for the determ ination of total alkaloid of Gelsemium elegans Benth.The influence of various affecting the result of determ ination was investigated.The content of total alkaloid of Gelsemium elegans benth was determined by bromecresol green.It could be concluded that this is a specific,quick and simple method for the determ ination of total alkaloid of Gelsemium elegans Benth.
Keywords:Gelsemium elegans Benth  total alkaloid  bromophenol blue colorimetry  
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