水稻灌浆期叶鞘蛋白质差异表达分析

为研究叶鞘在籽粒灌浆期间代谢的分子机理, 应用差异蛋白质组学方法分析了大穗型水稻“金恢809”在籽粒灌浆不同时期, 叶鞘的蛋白质组变化趋势, 共检测到23个差异蛋白质点, 其中11个得到鉴定, 并按其表达丰度的变化分为6类。第1类, 随着灌浆进程表达量逐渐降低, 如α-亚基草酰乙酸脱羧酶; 第2类, 表达量先升后降, 如二磷酸核酮糖羧化酶小链和ADP-核糖基化因子１; 第3类, 表达量先降后升再降低, 如生长素响应因子、锌指-C3HC4型家族蛋白、液泡H+-ATP酶和二磷酸核酮糖羧化酶/加氧酶活化酶; 第4类, 表达量先升后降再升又降, 如核酮糖α亚基结合蛋白; 第5类, 表达量先降后升, 如金属硫蛋白II相似蛋白-1A和牻牛儿基牻牛儿基二磷酸合酶; 第6类, 表达量逐渐升高, 如蛋白激酶家族蛋白。这6类蛋白分别参与叶鞘光合作用、激素调节、物质转运、植株衰老的抗性反应以及细胞信号转导, 共同调控叶鞘的源、库、流转化。

Analysis of Differential Expression of Proteins in Rice Leaf Sheath during Grain Filling

To reveal the molecular mechanism of metabolism in rice leaf sheath, this study investigated differential expression of proteins in rice leaf sheath of Jinhui 809 cultivar with large panicles and high setting percentage during grain filling by the method of differential proteomics. Twenty-three proteins with obviously different abundance were detected, and eleven of them were identified in function. Those functional proteins were classified into six categories according to their dynamic pattern of expression during grain filling. The first type showed down-regulation during grain filling, such as oxaloacetate decarboxylase alpha subunit. And the second one showed up-regulation at first and then down-regulation, such as ribulose bisphosphate carboxylase small chain and ADP-ribosylation factor 1. The third one was that down-regulated first, and then up and down regulated alternatively, such as auxin response factor, zinc finger, C3HC4 type family protein, vacuolar proton-ATPase, ribulose bisphosphate carboxylase/oxygenase activase. The forth type included rubisco binding-protein alpha subunit, showing an expression pattern of up and down regulation alternatively twice during grain filling. The fifth type performed a decreasing at first and then gradually increasing expression pattern , such as class II metallothionein-like protein 1A and geranylgeranyl diphosphate synthase. The sixth type was protein kinase family protein performing a gradually increasing expression pattern. These six different types of proteins involve in photosynthesis, hormone regulation, substance transportation, resistance response to plant senescence and cell signal diversion in leaf sheath respectively. They regulate the mechanism of sink to source transition together.