应用微卫星标记定位水稻恢复系密阳46的主效和微效恢复基因

 应用由704个株系组成的珍汕97A/(珍汕97B/密阳46)F6测交群体，针对水稻第10染色体和第1、11染色体短臂构建了微卫星标记连锁图谱，检测到控制野败型细胞质雄性不育育性恢复的4个QTL，其中位于第10染色体长臂中下部的Rf4具有主效效应，位于第1染色体短臂的Rf3具有较大效应，位于第10染色体长臂近着丝粒处的qRf10和第11染色体短臂近着丝粒处的qRf11表现出微效作用。研究还表明，在主效基因Rf4存在时，其他3个基因仍具有提高结实率的作用，但在Rf3和Rf4同时存在时，qRf10和qRf11的效应不明显。

SSR Marker-Based Mapping of Fertility-Restoring Genes in Rice Restorer Line Milyang 46

A SSR marker based linkage map covering chromosome 10 and the short arms of chromosomes 1 and 11 of rice was constructed and applied for mapping fertility-restoring genes in Zhenshan 97A/(Zhenshan 97B/Milyang 46)F_ 6 population consisting of 704 lines. Four QTLs were detected, among which Rf4 on the long arm of chromosome 10 displayed a major effect, Rf3 on the short arm of chromosome 1 had a considerable effect, and qRf10 and qRf11 in the pericentromeric regions of chromosomes 10 and 11, respectively, had minor effects. It was also found that Rf3, qRf10 and qRf11 contributed to higher spikelet fertility in the presence of the major gene Rf4, whereas qRf10 and qRf11 showed little effect in the presence of both Rf3 and Rf4.