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植物激素对朱砂根愈伤组织生长的影响
引用本文:何雪娇,赖钟雄.植物激素对朱砂根愈伤组织生长的影响[J].江西农业学报,2012,24(1):5-8.
作者姓名:何雪娇  赖钟雄
作者单位:1. 福建农林大学园艺植物生物工程研究所,福建福州350002;福建省热带作物科学研究所,福建漳州363001
2. 福建农林大学园艺植物生物工程研究所,福建福州,350002
基金项目:国家科技支撑计划项目,福建省重大科技平台建设项目
摘    要:采用单因素试验和正交试验的方法,研究了2,4-D、6-BA、NAA、KT等激素及其不同浓度组合对朱砂根愈伤组织生长和增殖的影响。结果表明:2,4-D对朱砂根愈伤组织生长的影响最大,KT的影响次之;在培养基MS+2,4-D 0.5mg/L中朱砂根愈伤组织的增殖系数最高,在正交试验筛选出的最佳培养基MS+2,4-D 0.1 mg/L+NAA 0.1 mg/L+KT 0.01mg/L中的次之。

关 键 词:朱砂根  植物激素  愈伤组织  增殖系数

Effect of Phytohormone on Callus Growth of Ardisia crenata
HE Xue-jiao , LAI Zhong-xiong.Effect of Phytohormone on Callus Growth of Ardisia crenata[J].Acta Agriculturae Jiangxi,2012,24(1):5-8.
Authors:HE Xue-jiao  LAI Zhong-xiong
Institution:1(1.Institute of Horticultural Plant Bioengineering,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2.Fujian Institute of Tropical Crop Sciences,Zhangzhou 363001,China)
Abstract:The growth and proliferation of the callus of Ardisia crenata under the conditions of different phytohormones(2,4-D,6-BA,NAA,KT etc.) and their different concentration combinations were studied by using single-factor test and orthogonal test methods.The results showed that 2,4-D had the greatest impact on the callus growth of Ardisia crenata,followed by KT.The multiplication coefficient of the callus of Ardisia crenata in culture medium MS+2,4-D 0.5 mg/L was maximum,followed by culture medium MS+2,4-D 0.1 mg/L+NAA 0.1 mg/L+KT 0.01 mg/L,which was screened out by orthogonal test.
Keywords:Ardisia crenata  Phytohormone  Callus  Multiplication coefficient
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