| 硫酸化黄芪多糖对CEF生长及抵抗IBDV感染的影响 |
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| 引用本文： | 黄小燕,胡元亮,王德云,王君敏,赵晓娜,张帆,郭振环,卢宇,孙峻岭.硫酸化黄芪多糖对CEF生长及抵抗IBDV感染的影响[J].南京农业大学学报,2009,32(1). |
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| 作者姓名： | 黄小燕 胡元亮 王德云 王君敏 赵晓娜 张帆 郭振环 卢宇 孙峻岭 |
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| 作者单位： | 南京农业大学中兽医学研究室,江苏南京,210095 |
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| 基金项目： | 国家自然科学基金，江苏省普通高校研究生科研创新计划 |
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| 摘 要： | 采取一步醇沉和分步醇沉法提取4种黄芪多糖APS,、APS10、APS50和APS50,用氯磺酸-吡啶法进行硫酸化修饰,得到4种硫酸化黄芪多糖(sAPS) sAPSt、sAPS40、sAPS50和sAPS60,用MTT法比较了4种硫酸化黄芪多糖对鸩胚成纤维细胞(CEF) 的生长及抵抗鸡传染性法氏囊病毒(IBDV) 感染的影响,以末修饰的APSt为对照.结果显示,APS1显著促进CEF生长,细胞毒性较低,但抵抗鸡传染性法氏囊病毒感染细胞的作用较弱;sAPS抵抗鸡传染性法氏囊病毒感染的作用显著增强,显效浓度降低,但安全浓度也降低.表明通过硫酸化修饰可以提高黄芪多糖的抗病毒活性,但高浓度sAPS的细胞毒性增强.
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| 关 键 词： | 黄芪多糖 硫酸化修饰 鸡胚成纤维细胞 抗IBDV感染 |
Effect of sulfated astragalus polysaccharides on growth of chicken embryo fibroblast and cellular infectivity of infectious bursal disease virus |
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| HUANG Xiao-yan,HU Yuan-liang,WANG De-yun,WANG Jun-min,ZHAO Xiao-na,ZHANG Fan,GUO Zhen-huan,LU Yu,SUN Jun-ling.Effect of sulfated astragalus polysaccharides on growth of chicken embryo fibroblast and cellular infectivity of infectious bursal disease virus[J].Journal of Nanjing Agricultural University,2009,32(1). |
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| Authors: | HUANG Xiao-yan HU Yuan-liang WANG De-yun WANG Jun-min ZHAO Xiao-na ZHANG Fan GUO Zhen-huan LU Yu SUN Jun-ling |
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| Institution: | Institute of Traditional Chinese Veterinary Medicine;Nanjing Agricultural University;Nanjing 210095;China |
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| Abstract: | Four kinds of astragalus polysaccharides(APS),APSt,APS40,APS50 and APS60,were extracted by one-step and stepwise ethanol precipitation method and modified by chlrulfonic-pyridine method to obtain four sulfated APS(sAPS),sAPSt,sAPS40,sAPS50,sAPS60,respectively.The effects of four sAPS on growth of chicken embryo fibroblast(CEF)and cellular infectivity of infections bursal disease virus(IBDV)were compared by MTT method taking non-modified APSt as control.The results showed that non-modified APSt promoted CEF ... |
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| Keywords: | astragalus polysaccharide sulfated modification CEF resisting IBDV infection |
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