FecB基因在5个中国地方绵羊品种中的多态性及其与产羔数的关联分析

为解析中国地方绵羊品种产羔数差异形成的遗传学机制,以5个不同繁殖力的中国地方绵羊品种(湖羊、藏羊、蒙古羊、阿勒泰羊和多浪羊)为研究对象,利用PCR-RFLP技术检测FecB基因的多态性,比较不同繁殖力群体间FecB的分布情况,并与其产羔数进行关联分析。结果显示:湖羊群体中存在BB、B+、++三种基因型,藏羊、蒙古羊及阿勒泰羊群体中仅存在B+和++两种基因型,多浪羊群体中仅存在++基因型。湖羊、藏羊、蒙古羊、阿勒泰羊及多浪羊群体中BB、B+及++基因型频率依次是0.69、0.28、0.03,0.00、0.13、0.88,0.00、0.08、0.92,0.00、0.08、0.92和0.00、0.00、1.00;B等位基因频率由高到低依次为湖羊>藏羊>蒙古羊=阿勒泰羊>多浪羊。湖羊、藏羊、蒙古羊和阿勒泰羊中该位点He和PIC分别为0.29、0.25,0.12、0.11,0.08、0.07和0.08、0.07,且在以上4个品种中BB和B+基因型个体产羔数比++基因型个体高(P<0.01),BB基因型个体和B+基因型个体之间无差异(P>0.05)。由此可推断,FecB或许是决定绵羊产羔数的主效基因,亦或是与其存在密切相关的标记基因,可助力绵羊产羔数性状MAS技术和为多胎绵羊新品种(系)培育提供素材。

Genetic Polymorphism of FecB Gene and Effect in Five Chinese Local Sheep Breeds

This study compared the polymorphism of FecB gene in the Hu sheep,Tibetan sheep,Mongolian sheep,Duolang sheep and Altai sheep,and analyzed the relation between different fecundity and genotype,which would provide a theoretical basis for marker-assisted selection and breeding for prolificacy in sheep.The gene polymorphism of FecB gene was analyzed by PCR-RFLP to compare the differences between the groups of different fertility.The results showed that there were three genotypes(BB,B+and++)in Hu sheep,while two genotypes(B+and++)were found in Tibetan sheep,Mongolian sheep and Altai sheep,and only++genotype existed in Duolang sheep.The genotype frequency of BB,B+and++in Hu sheep,Mongolian sheep,Altai sheep and Duolang sheep were 0.69,0.28,0.03;0.00,0.13,0.88;0.00,0.08,0.92;0.00,0.08,0.92 and 0.00,0.00,1.00 respectively.B allele frequency from high to low in the order was Hu sheep>Tibetan>Mongolian=Altai>Duolang.In addition,the He and PIC for Hu sheep,Tibetan sheep,Mongolian sheep and Altai sheep were 0.29,0.25;0.12,0.11;0.08,0.07 and 0.08,0.07.And in the above four varieties of BB and B+individual lambing number was significantly higher than++genotype(P<0.01),BB and B+no difference between individuals,FecB gene might be a major gene which affected the prolificacy trait of sheep or it was regarded as a genetic makers closely linked to target gene and could be as a marker assisting selection and breeding of improve fecundity.