杉木茎尖诱导愈伤组织及植株再生研究

用杉木优良单株基部萌蘖条茎尖作为外植体进行组培快繁试验，结果表明：愈伤组织在改良MS＋KT0．2mg／L＋2，4-D1．0mg／L培养基上，外植体诱导率为86．7％；不定芽分化与增殖培养基为改良MS＋KT1．0mg／L＋IBA0．5mg／L，分化系数达3．42；芽苗培育约30d后，用1／2MS＋IBA0．2mg／L＋NAA0．5mg／L作生根培养基培养约25d，生根率可达93％以上。生根苗移植到覆盖3cm厚黄心土的苗床上栽培，成活率可达90％以上。

Study on Callus Induction and Plant Regeneration by Shoot Tip of Cunninghamia lanceolata

The shoot tips sprouted from the trunk base of the superior individual plants of Cunninghamia lanceolata were applied as the explants to induce the callus on the improved medium MS ＋ KT 0. 2 mg/L ＋ 2,4 - D 1.0 mg/L. The results showed that the induction rate reached 86.7% ; the culture medium for differentiation and multiplication of adventitious bud was the improved MS ＋ KT 1.0 mg/L ＋ IBA 0.5mg/L,whose differentiation coef- ficient reached 3.42. The culture medium for rooting to be applied 30 days later was the improved 1/2MS ＋ IBA 0. 2 mg/L ＋ NAA 0. 5 mg/L, by which the rooting rate was 93% within 25 days. The rooted seedlings were trans- planted to the seedbed covered by 3 cm yellow soil for further cultivation, and the transplanting survival rate was over 90%.