紫皮石斛组培技术的优化研究

以紫皮石斛种子和茎段作为外植体,研究芽苗增殖培养、生根培养及组培苗优化条件。结果表明:种子最适宜作为快繁的外植体,种子通过无菌小芽苗、芽苗增殖、壮苗生根的途径再生成完整植株;并探索出适宜的培养基配方:初代诱导培养基为1/2MS+1mg/L BA+0.5mg/L NAA,种子直接萌发长成大量绿色、长势整齐的无菌小芽苗;增殖培养基为MS+2mg/L BA+0.5mg/L NAA+5%马铃薯汁+5%香蕉泥,芽苗增殖倍数高,达到4.6倍,且长势好、芽苗粗壮;生根壮苗培养基为1/2MS+0.5mg/L NAA+10%香蕉泥+0.1%活性炭,生根苗平均根数和根长达到了最佳状态,根系发达,苗高、健壮,叶色浓绿。培养基中适宜的琼脂浓度为6g/L,增殖倍数较高,植株直立,叶片也不弯曲。

Optimizing Study on Tissue Technology of the Purple Skin Dendrobe

With the purple skin Dendrobe seed and stem section as explants,the bud seedling proliferation,rooting,and optimized conditions of plantlets were studied.The results showed that the seed was most appropriate as rapid propagation explant,seeds through the small bud seedling,the proliferation and seedling rooting of the way into complete plant regeneration;And a suitable culture medium formula was explored:beginning the generation of inductive medium for 1/2MS+1 mg/L BA+0.5 mg/L NAA,seed germination directly grow,growth of large green and sterile small bud seedling;Proliferation medium for MS+2 mg/L BA+0.5 mg/L NAA+5% potatoes juice+5% mashed bananas,bud seedling multiple of 4.6 times as high proliferation and growth better,the bud seedling stout;Rooting medium 1/2MS+0.5 mg/L NAA+10% mashed bananas+0.1% activated carbon,take root seedlings root number and root for average to best,root system developed,seedling height,robust,and the leaves deep green.Medium suitable for the AGAR concentration of 6 g/L,therefore,higher multiples,erect plant,leaf also didn’t bend.