| 基于IGS序列的两株蜜环菌分子鉴定 |
| |
| 引用本文： | 冯健,范俊岗,金若忠,陈罡.基于IGS序列的两株蜜环菌分子鉴定[J].北方园艺,2012(3):116-118. |
| |
| 作者姓名： | 冯健 范俊岗 金若忠 陈罡 |
| |
| 作者单位： | 辽宁省林业科学研究院,辽宁沈阳10032 |
| |
| 基金项目： | 辽宁省科技支撑计划资助项目 |
| |
| 摘 要： | 以从猪苓Polyporus umbellatus(Pers.)Fr.]菌核和榛蘑(Armillariella mellea)子实体中分离得到菌株bs和zm为试材,通过对其IGS区段进行分析并结合分离材料和培养菌丝的形态特征确定其分类学地位。结果表明:菌核和子实体的生境及形态学特征初步鉴定为蜜环菌;对2个菌株的分类学地位进行了分析确定,并利用引物5SA和CNL12扩增其IGS区域,进行BLAST比对分析,菌株bs和zm均为高炉蜜环菌,为辽宁省猪苓的伴生菌。
|
| 关 键 词： | 蜜环菌 PCR rDNA |
Molecular Identification of Two Armillaria Based on IGS Sequence Analysis |
| |
| FENG Jian
,
FAN Jun-gang
,
JIN Ruo-zhong
,
CHEN Gang.Molecular Identification of Two Armillaria Based on IGS Sequence Analysis[J].Northern Horticulture,2012(3):116-118. |
| |
| Authors: | FENG Jian FAN Jun-gang JIN Ruo-zhong CHEN Gang |
| |
| Institution: | (Liaoning Academy of Forestry Sciences,Shenyang,Liaoning 110032) |
| |
| Abstract: | The bs and zm was isolated from sclerotia of Polyporus Umbellatus and body of Armillaria mellea respectively as test material.Through the IGS Section was analyzed and combinated the morphological characteristics of mycelium of separation material,the taxonomic position were determined.The results showed that the habitats and morphological features of sclerotia of Polyporus Umbellatus and body of Armillaria mellea,two asociated fungi were identified as Armillaria preliminarily.In order to confirm the taxonomic position of two Armillarias,PCR with 5SA and CNL12 as primers was used to amplify rDNA of two Armillarias.The results showed that both of Armillarias were Armillaria gallica,which was the associated fungus of Polyporus Umbellatus in Liaoning Province. |
| |
| Keywords: | Armillaria PCR rDNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
