| 宁夏枸杞叶片组培快繁技术研究 |
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| 引用本文： | 郑国琴,柳金凤.宁夏枸杞叶片组培快繁技术研究[J].北方园艺,2012(1):122-124. |
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| 作者姓名： | 郑国琴 柳金凤 |
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| 作者单位： | 1. 宁夏大学生命科学学院,宁夏银川750004;宁夏林业研究所股份有限公司种苗生物工程国家重点实验室,宁夏银川750004
2. 宁夏林业研究所股份有限公司种苗生物工程国家重点实验室,宁夏银川,750004 |
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| 基金项目： | 宁夏自治区科技攻关资助项目(S20116400004) |
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| 摘 要： | 以宁夏枸杞的叶片为外植体,进行愈伤组织诱导、不定芽诱导、分化、试管苗生根、移栽的研究。结果表明:MS+NAA 0.2mg/L+6-BA 1.0mg/L是叶片愈伤组织和不定芽诱导的最佳培养基;MS+6-BA 0.3mg/L+IAA 0.8mg/L是试管苗分化的最佳培养基;1/2MS+IBA0.6mg/L+NAA 0.2mg/L是试管苗生根的最佳培养基。
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| 关 键 词： | 枸杞 外植体 叶片 组织培养 |
Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L. |
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| ZHENG Guo-qin
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LIU Jin-feng.Study on Rapid Propagation by Leaf Tissue Culture of the Lycium barbarum L.[J].Northern Horticulture,2012(1):122-124. |
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| Authors: | ZHENG Guo-qin LIU Jin-feng |
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| Institution: | 1(1.College of Life Science,Ningxia University,Yinchuan,Ningxia 750004;2.Seedlings Biological Engineering State Key Laboratory of Ningxia Forestry Research Institute Limited Company,Yinchuan,Ningxia 750004) |
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| Abstract: | The leaf of Lycium barbarum L.was used as explants,induction of the callus and adventitious buds,differentiation,taking roots and transplant were studied.The results showed that the best media for inducing callus and adventitious buds was MS+NAA 0.2 mg/L+6-BA 1.0 mg/L;the best media for differentiation was MS+6-BA 0.3 mg/L+IAA 0.8 mg/L;the best media for inducing roots was 1/2MS+IBA 0.6 mg/L+NAA 0.2 mg/L. |
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| Keywords: | wolfberry explant leaves tissue culture |
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