羊肚菌M延-5菌株菌丝体生长所需碳氮源的研究

M延-5是从陕北南泥湾野生浅黄色羊肚菌菌盖中分离筛选的菌株。用不同碳、氮源对其分别进行固体、液体培养。结果表明:最佳碳源,固体培养为葡萄糖,液体培养为麦芽糖;最佳氮源,固体培养为尿素,液体培养为蛋白胨。方差分析结果表明:固体培养菌丝体长速,碳源中蔗糖显著快于葡萄糖、蔗糖,麦芽糖和淀粉极显著快于乳糖和甘露醇;氮源中酵母粉显著快于尿素和硝酸钾,前3种极显著快于蛋白胨、硝酸铵和硫酸铵。液体培养菌丝体干重,碳源中麦芽糖、淀粉、葡萄糖极显著高于蔗糖、乳糖和甘露醇;氮源中蛋白胨、尿素、酵母粉极显著高于硝酸钾、硝酸铵和硫酸铵。

Research on the Growing Resources of Toadstool M.sp(Yan-5) Bacterial Strain and Mycelium Needed for Nutrition, Carbon and Nitrogen

M.sp(Yan-5) is separated and selected from the pileus of light yellow wild toadstool that grows in Nanniwan,northern of Shaanxi province.We tested different carbon and nitrogen to cultivate toadstool M.sp(Yan5) separately in solid and liquid.The experiment result showed:the best carbon source in solid was glucose and in liquid was maltose;the best nitrogen source was carbamide in solid and was peptone in liquid.Variance analysis concluded: as for growth speed of mycelium cultivate in solid,sucrose in carbon nutrition is obviously quicker than glucose,sucrose and maltcose.And amylum was terribly quicker than lactose and mannitol.Yeast powder in nitrogen was quicker than carbamide and potassium nitrate.In short,the previous three elements: sucrose in carbon nutrition,amylum and yeast powder were greatly quicker than peptone were ammonium and vitriol.Variance analysis also showed as dry weight of mycelium cultivated in liquid,maltcose,amylum and glucose in carbon notably higher than sucrose,lactose and mannitol;peptone,carbamide and yeast powder in nitrogen were greatly higher than potassium nitrate,ammonium nitrate and ammiaonia sulfate.