OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
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Authors: | CAO Peng-hui WANG Di GAO Su LIU Xi QIAO Zhong-ying XIE Yu-lin DONG Ming-hui DU Tan-xiao ZHANG Xian ZHANG Rui JI Jian-hui |
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Institution: | 1. Institute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.China;2. Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai''an 223001, P.R.China;3. School of Life Sciences, Huaiyin Normal University, Huai''an 223300, P.R.China;4. Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional Cooperative Innovation Center for Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai''an 223300, P.R.China |
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Abstract: | Plant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice. OsDXR knock-out lines displayed the albino phenotype and could not complete the whole life cycle process. OsDXR was highly expressed in rice leaves, and subcellular localization indicated that OsDXR is a chloroplast protein. Many genes involved in chlorophyll biosynthesis and chloroplast development were differentially expressed in the OsDXR knock-out lines compared to the wild type. Moreover, we found that the RNA editing efficiencies of ndhA-1019 and rpl2-1 were significantly reduced in the OsDXR knock-out lines. Furthermore, OsDXR interacted with the RNA editing factor OsMORF1 in a yeast two-hybrid screen and bimolecular fluorescence complementation assay. Finally, disruption of the plastidial 2-C-methyl-derythritol-4-phosphate pathway resulted in defects in chloroplast development and the RNA editing of chloroplast genes. |
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Keywords: | rice OsDXR Chloroplast development RNA editing OsMORF1 |
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