羊胎盘转移因子注射液对大白鼠和幼犬营养作用的试验研究

目的]通过研究羊胎盘转移因子注射液对健康大白鼠、人工创伤模型大白鼠和健康杂种幼犬的血浆总蛋白(TP)、白蛋白(ALB)、超氧化物歧化酶(SOD)、一氧化氮(NO)和血红蛋白(Hb)含量的影响,探讨供试药品对受试动物的营养作用,进而评估其临床营养功效。方法]将24只健康大鼠和16只健康杂种幼犬分别随机分为试验组和对照组,为试验组大鼠和试验组幼犬灌服供试药品,并检测给药前后血浆TP、ALB和Hb含量的变化。将45只健康大鼠人工创伤造模后分成低剂量试验组、中剂量试验组和对照组3个组,试验组大鼠使用不同剂量供试药品,于术后3、7、14d测定血浆TP、ALB、SOD和NO含量的变化。结果]①为健康大鼠灌服供试药品后,与对照组相比,试验组健康大鼠血浆TP、ALB含量显著(P0.05)提高。②为健康杂种幼犬灌服供试药品后3d,与对照组相比,试验组健康杂种幼犬血浆中TP、Hb含量均极显著(P0.01)提高,ALB含量显著(P0.05)提高。③为人工创伤模型大鼠灌服供试药品后,与对照组相比,各试验组术后3d血浆TP、ALB、NO含量均提高;术后7 d中剂量组血浆TP、ALB、NO含量均极显著提高(P0.01),低剂量组显著提高(P0.05);术后14 d,试验组血浆TP、ALB含量恢复到术前,试验组血浆NO含量升高,无显著差异(P0.05)。与对照组相比,术后3 d和7 d,各试验组血浆SOD含量升高,无显著差异(P0.05);术后14d,各试验组SOD含量升高,中剂量组显著(P0.05)升高。结论]羊胎盘转移因子注射液可显著改善健康大鼠和健康杂种幼犬的营养状况,促进术后大鼠创伤恢复和幼犬的生长发育,提高机体抗病能力,在临床上兼具改善营养和提高免疫力的作用。

Experimental Study on the Nutritional Effects of Sheep Placental Transfer Factor Injection on Rats and Young Canine

Objective] The research aimed to study effects of sheep placental transfer factor （SPTF） on the contents of total protein（TP）, albumin（ALB）, superoxide dismutase （SOD）,nitric oxide（NO） and hemoglobin（Hb） in the plasm of healthy rats, artificial wound model rats and young healthy hybrid canine. The effects of test drugs on nutritional situations of test animals were discussed to evaluate the clinic nutritional efficacy. Method] 24 healthy rats and 16 young healthy hybrid canine were randomly divided into test group and control group. Rats in test group and young canine in test group were administrated with the test drug to detect the content changes of plasma TP,ALB and Hb before and after drug administration. 45 healthy rats after artificial wound modeling were divided into 3 groups （low-dose test group, medium-dose test group and control group）. Rats in test groups were administrated with different dose of test drug. And the content changes of TP, ALB, SOD and NO in the plasma after operation 3, 7 and 14 days were determined. Result]①After healthy rats were administrated with test drug, the contents of TP and ALB in the plasma of healthy rats in test group were significantly increased than that in control group（P〈0.05）.②After healthy young hybrid canine were administrated with test drug three days, the content of TP and Hb in the plasma of healthy young hybrid canine in test group were extremely significantly increased than control group（P〈0.01）, and ALB content was significantly increased（P〈0.05）.③After artificial wound model rats were administrated with test drug, the contents of TP, ALB and NO in the plasma in test groups were increased after operation 3 days. After operation 7 days, the contents of TP, ALB and NO in the plasma were extremely significantly increased in medium-dose test group （P〈0.01） and significantly increased in low-dose test group （P〈0.05）. After operation 14 days, the contents of TP and ALB in the plasma in test groups recovered to the level before operation, NO content in the plasma in test groups increased, but there was no significant difference （P〉0.05）. Compared with control group, SOD content in the plasma in test groups increased after operation 3 and 7 days, but there was no significant difference （P〉0.05）. After operation 14 days, SOD content in the plasma in test groups increased and SOD content in medium-dose group was significantly increased（P〈0.05）. Conclusion] Sheep placental transfer factor could significantly improve the nutritional situations of healthy rats and healthy young hybrid canine, promote the wound recovery of rats after operation and the growth and development of young canine and enhance the disease resistance. And SPTF had the roles of improving the nutrition and enhancing the immunity.