油茶12个物种花药愈伤组织诱导及继代培养

选择12个油茶物种进行花药愈伤组织的诱导,以小果油茶、普通油茶、短柱油茶、浙江红花油茶、茶陵红花油茶、多齿红花油茶诱导率较高,在40％～70％之间,其中小果油茶、普通油茶的愈伤组织诱导率高达70％。红皮糙果油茶、广西糙果油茶、茶陵红花油茶、多齿红花油茶、攸县油茶花药褐化较严重。继代培养中6-BA和KT均有较好的促进作用,小果油茶、普通油茶、短柱油茶、浙江红花油茶、茶陵红花油茶、多齿红花油茶的愈伤组织均增殖较好。油茶花药培养应以小果油茶、普通油茶、短柱油茶、浙江红花油茶、茶陵红花油茶、多齿红花油茶作为进一步试验的基本材料,开展下一步的愈伤组织增殖和脱分化试验。

Anther Callus Induction and Subculture for Twelve Species in Camellia

Taken twelve oil-tea species as materials, anther callus was inducted. The rates of induction anther callus from Camellia oleifera Abel. , C. meiocarpa Hu.,C. brevistyla, C. chekiangoleosa Hu. , C pbellocapsa, C. polyodondta Hu. were higher than the others, from 40% to 70%;those from C. oleifera Abel.and C. meiocarpa Hu. were up to 70%. Anthers from C. crapnelliana Tutchher, C. furfuracea, C. pbellocapsa,C. polyodondta Hu. , C. yuhsiensis browned seriously. In subculture, the callus of C. oleifera Abel. ,C. meiocarpa Hu. , C. brevistyla, C. chekiangoleosa Hu. , C. pbellocapsa,C. polyodondta Hu. grew well; 6-BA and KT played better role in subculture. The further experiments on callus multiplication and secondary differentiation could take Camellia oleifera Abel.,C. meiocarpa Hu. , C. brevistyla, C. chekiangoleosa Hu. , C. pbellocapsa, C. polyodondta Hu. as materials.