玉米自交系幼胚高效再生系统的建立

以22份玉米自交系为试验材料,以幼胚为外植体,研究基因型、诱导（分化）培养基、继代次数、Ag-NO3浓度对玉米幼胚胚性愈伤组织的诱导、保持和植株分化的影响。结果表明,玉米幼胚培养能力受基因型、培养基及二者互作效应的影响,基因型是主要的影响因子。3 mg/L的2,4-D对胚性愈伤组织诱导和保持起关键作用;0.5 mg/L 6-BA抑制胚性愈伤组织的发生;8-12 mg/L AgNO3明显改善部分基因型愈伤组织质量,提高胚性愈伤组织诱导率。分化培养基中添加1 mg/L的KT和0.5 mg/L的IBA促进绿苗的分化和根系的生长。胚性愈伤组织继代3-5次后调整到最佳状态。确定了玉米幼胚培养胚性愈伤组织诱导的最佳培养基为N6＋3 mg/L 2,4-D＋8-12 mg/L AgNO3＋0.2 mg/L IAA,最佳分化培养基为N6＋1 mg/L KT＋0.5 mg/L IBA。

Establishing on High Efficiency Regeneration System of Maize Inbred Immature Embryo

22 maize genotypes were used to study the influence of genotypes,media,AgNO3 and subculture time on induction and plant regeneration of embryogenic callus in immature embryo culture.The results showed that the embryogenic callus induction and regenerating plants were affected by genotype,medium(hormone) and the interaction between them.Genotype was the crucial factor.Addition of 3 mg/L 2,4-D played an impotant role in embryogenic callus induction.0.5 mg/L 6-BA added in induction media lowed callus quality.8~12 mg/L AgNO3 could improved callus quality from partial genotypes.1 mg/L KT and 0.5 mg/L IBA added increased plant regenerating and rooting.Frequency embryogenic callus induction and regenerating plant was higher for subculture 3-5 times.The optimum medium for calls induction was N6 3 mg/L 2,4-D 8~12 mg/L AgNO3 0.2 mg/L IAA.The optimum medium for plant regeneration was N6 1 mg/L KT 0.5 mg/L IBA.