| 刺五加体细胞胚状体发生及成苗的研究 |
| |
| 引用本文： | 郑颖,张利萍,方海霞,白荣芬,丁琳琳,郑璐.刺五加体细胞胚状体发生及成苗的研究[J].辽宁林业科技,2011(2):8-9,13. |
| |
| 作者姓名： | 郑颖 张利萍 方海霞 白荣芬 丁琳琳 郑璐 |
| |
| 作者单位： | 1. 辽宁省森林经营研究所,辽宁丹东,118002
2. 辽宁省干旱地区造林研究所,辽宁朝阳,122000 |
| |
| 摘 要： | 以刺五加试管苗叶片为试材,将叶片接种于含有不同种类和浓度激素的培养基上进行培养,研究不同激素对刺五加胚性愈伤组织诱导、胚状体发生及生根的影响。结果表明：诱导叶片胚性愈伤组织发生的最适培养基为MS＋6-BA 1.5 mg.L-1＋2,4-D 1.0 mg.L-1,胚状体生长发育的最适培养基为MS＋6-BA1.0 mg.L-1＋IBA0.5 mg.L-1,生根的最适培养基为1/2MS＋NAA0.4 mg.L-1。
|
| 关 键 词： | 刺五加 胚状体 成苗 |
Research on somatic embryogenesis and seedling formation regulation of Acanthopanax senticosus |
| |
| ZHENG Ying,ZHANG Li-ping,FANG Hai-xia,BAI Rong-fen,DING Lin-lin,ZHENG Lu.Research on somatic embryogenesis and seedling formation regulation of Acanthopanax senticosus[J].Liaoning Forestry Science and Technology,2011(2):8-9,13. |
| |
| Authors: | ZHENG Ying ZHANG Li-ping FANG Hai-xia BAI Rong-fen DING Lin-lin ZHENG Lu |
| |
| Institution: | ZHENG Ying1,ZHANG Li-ping1,FANG Hai-xia1,BAI Rong-fen1,DING Lin-lin1,ZHENG Lu2(1.Liaoning Institute of Forest Management,Dandong 118002,China,2.Afforestation Institute of Liaoning in Arid Zones,Chaoyang 122400,China) |
| |
| Abstract: | The effect of plant growth regulating substances on embryonic callus induction,somatic embryogenesis and rootting were investigated by means of vitro culture from leaf of test-tube seedlings of Acanthopanax senticosus.The results showed that the optimum medium on embryogenic callus induction was MS＋6-BA 1.5 mg·L-1＋2,4-D 1.0 mg·L-1.The optimum medium on growth and development of somatic embryo was MS＋6-BA 1.0 mg·L-1＋IBA 0.5 mg·L-1.The optimum medium on rootting was1/2MS＋NAA 0.4 mg·L-1. |
| |
| Keywords: | Acanthopanax senticosus somatic embryo seedling |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
