小簇麦易位系V9128-3抗条锈病基因的遗传分析和SSR分子标记

 用7个我国当前流行的条锈菌生理小种对V9128-3的抗条锈性进行了评价,表明本易位系对我国优势流行小种具有良好的抗病性。以Su-4对V9128-3与铭贤169配置的F₁、BC₁F₁、F₂及F₃代群体进行了遗传分析,并对其中1个F₂群体进行了SSR标记,再用BC₁F₁群体的部分单株和F₃家系进行连锁标记的初步验证。遗传分析表明了V9128-3对Su-4的抗病性由1对显性核基因独立控制,从219对SSR引物中筛选到2个位于2AL上的该基因YrHV(暂命名)两侧的标记Xgwm356和Xwmc658,遗传距离分别为8.5和5.6cM,所用部分BC₁F₁单株和F₃家系验证了该2个标记与YrHV连锁性。将此标记可用于小麦抗条锈病分子标记辅助育种。

Genetic analysis and SSR molecular mapping of translocation line V9128-3 derived from Triticum aestivum-Haynaldia villosa resistance to stripe rust

The Triticum aestivum-Haynaldia villosa translocation line V9128-3 was inoculated with seven dominant races of Puccinia striiforms f. sp. tritici prevalent in China, the results of resistance evaluation showed that the translocation line V9128-3 possessed resistance to the current epidemic yellow rust races. V9128-3, susceptible cultivar Mingxian 169 and their progeny of F₁, F₂, BC₁F₁, F₃ were inoculated with race Su-4, one of the F₂ progeny were used for developing molecular markers of stripe rust resistence gene in V9128-3, a portion of BC₁F₁ individuals and F₃ lines were used to validate the the linked markers. The results of inheritance analysis showed that V9128-3 resistance to Su-4 was controlled by one dominant gene. Two markers Xgwm356 and Xwmc658 located on 2AL from 219 SSR primer combinations were found linked to YrHV (temporarily designated). YrHV was 8.5 cM from Xgwm356 and 5.6 cM from Xwmc658, respectively, the two sites linked to YrHV were validated by a portion of BC₁F₁ individuals and F₃ lines. These markers would be used for breeding new wheat cultivars with marker-assisted selection.