Chemical composition of the essential oil and antioxidant activity of methanolic extracts from fruits and flowers of Hypericum lydium Boiss. |
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Authors: | Tuba ?erbetç i,Nurten Ö zsoyBetü l Demirci,Ay?e Can?ü kran Kü ltü r,K.H. Can Ba?er |
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Affiliation: | a Istanbul University, Faculty of Pharmacy, Department of Pharmacognosy, 34116 Beyaz?t, Istanbul, Turkey b Istanbul University, Faculty of Pharmacy, Department of Biochemistry, 34116 Beyaz?t, Istanbul, Turkey c Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470 Eski?ehir, Turkey d Istanbul University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 34116 Beyaz?t, Istanbul, Turkey e King Saud University, College of Science, Botany and Microbiology Department, 1145 Riyadh, Saudi Arabia |
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Abstract: | This study was conducted to evaluate the essential oil composition and antioxidant activity of methanolic extracts from fruits and flowers of Hypericum lydium Boiss., a wild growing species of the Turkish flora. The antioxidant activities were determined through several biochemical assays. Both extracts showed an inhibitory effect against the formation of TBARS in a phosphatidylcholine liposome model system, moderate scavenging effect on DPPH and superoxide radicals, prominent reducing power and inhibitory effect on deoxyribose degradation in both the nonsite and site-specific assay, but a greater hydroxyl radical scavenging activity was observed in the non-site specific assay, suggesting that the extracts were better at scavenging hydroxyl radicals than at chelating iron. Correlation analysis indicated that there was a linear relationship between antioxidant potency, free-radical scavenging activity, reducing power and the content of flavonoids of fruits and flowers extracts. The essential oils obtained by hydrodistillation were analyzed by GC and GC-MS. In total forty-three compounds were identified. The most abundant components were monoterpenes hydrocarbons represented principally by α-pinene. The tested oil showed no antioxidant activity. |
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Keywords: | Hypericum lydium Essential oil Antioxidant DPPH TBARS GC and GC-MS |
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