| 应用夹心阻断ELISA测定血清中抗狂犬病抗体的研究 |
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| 引用本文： | 李金中,夏平安,李佑民,周晓明,张国宪,侯世宽,侯安祖. 应用夹心阻断ELISA测定血清中抗狂犬病抗体的研究[J]. 中国兽医学报, 1992, 0(4) |
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| 作者姓名： | 李金中 夏平安 李佑民 周晓明 张国宪 侯世宽 侯安祖 |
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| 作者单位： | 兽医大学传染病教研室(李金中，夏平安，李佑民)，河南省南阳地区兽医防治站(周晓明，张国宪)，兽医大学军事兽医研究所(侯世宽)，河南省兽医防治站(侯安祖) |
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| 摘 要： | 本研究建立的检测狂犬病抗体的夹心阻断ELISA,直接利用未经提纯的病毒悬液代替提纯的抗原,并仅用一种酶标抗体,测定了9种动物的血清.本方法敏感度的95%可信限为0.0013～0.0071IU/mL,比小鼠中和试验和微量免疫酶试验均敏感.按阻断50%判定血清ELISA的阴阳性,9种动物的1134份血清中有91份阳性,其中发病点的牛、猪、犬、家鼠血清的阳性率均在10%以上.根据对一定量的抗原阻断率相同时,抗体浓度一致的原理,测定了7种动物的185份血清效价,结果狂犬病抗体浓度在0.01IU/mL以上的为63份.利用夹心阻断ELISA和小鼠中和试验测定了7种动物的23份血清,阳性份数分别为12和11.两种方法均证明家鼠血清中有狂犬病抗体.本研究表明,测定狂犬病抗体的夹心阻断ELISA,简便、敏感、快速,可用于流行病学调查.
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| 关 键 词： | ELISA 阻断 狂犬病 抗体 |
Detecting the Rabies Antibody in Serum by Sandwich Blocking ELISA |
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| Li Jinzhong et al. Detecting the Rabies Antibody in Serum by Sandwich Blocking ELISA[J]. Chinese Journal of Veterinary Science, 1992, 0(4) |
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| Authors: | Li Jinzhong et al |
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| Abstract: | A sandwich blocking ELISA (SB-ELISA) for detecting the rabies antibody in serum was developed with the virus suspension instead of the purified viral antigen. Its sensitivity of 95% confidence was 0. 0013 IU/mL of rabies antibody, which higher than those of mouse neutralization test and micro-ELISA. 50% blocking rate is recognized as the critical value of positive result. Of 1134 serum samples from 9 species of animals, the positive rates of cattle, swine, dogs and house mice of the villages in where rabies had been occurred were more than 10%. The presence of rabies antibody in sera of house mice was also confirmed with mouse neutralization test. It was demonstrated that the method developed is simple, sensitive and rapid and could be used in epizootic surveys. |
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| Keywords: | ELISA blocking rabies antibody |
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