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| 鹅细小病毒NS2基因的克隆和序列分析 | |
| 引用本文： | 阮二垒,陈芳艳,陈瑞爱,王林川,杨丽云.鹅细小病毒NS2基因的克隆和序列分析[J].中国动物检疫,2009,26(10):32-33. |
| 作者姓名： | 阮二垒 陈芳艳 陈瑞爱 王林川 杨丽云 |
| 作者单位： | 1. 广东大华农动物保健品股份有限公司,广东新兴,527400 2. 华南农业大学动物科学学院,广州,510642 3. 广东大华农动物保健品股份有限公司,广东新兴,527400;华南农业大学兽医学院,广州,510642 4. 华南农业大学兽医学院,广州,510642 |
| 摘 要： | 参照GenBank发表的GPVB株全基因序列,设计并合成一对引物,通过PCR技术扩增出GPVS1株NS2基因,目的片段长约1.4kb,将目的片段克隆到pMD18-T载体后进行序列测定和分析,结果表明:GPVS1株NS2基因全长1356bp,编码451个氨基酸,与国外已发表的GPVB株相比核苷酸序列同源性为98.89%,推导氨基酸序列同源性为98.89%。 |
| 关 键 词： | 鹅细小病毒 NS2基因 克隆 序列分析 |
Cloning and sequencing of NS2 gene of goose parvovirus |
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| RUAN Er-lei,Chen Fang-yan,Chen Rui-ai,WANG Lin-chuan,Yang Li-yun.Cloning and sequencing of NS2 gene of goose parvovirus[J].China Journal Of Animal Quarantine,2009,26(10):32-33. | |
| Authors: | RUAN Er-lei Chen Fang-yan Chen Rui-ai WANG Lin-chuan Yang Li-yun |
| Institution: | 1 Guangdong Dahuanong Animal Health Products Co. Ltd Xinxing 527400 China. 2 College of Animal Science,South China Agricultural University, Guangzhou 510642, China 3 College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China) |
| Abstract: | According to the sequence of GPVB strain published in GenBank , a pair of primers were designed by Primer5.0. NS2 gene of GPV is amplified by PCR. The gene of interest was cloned into the vector pMD18-T and sequenced. The results showed that NS2 gene consisted of 1356 nucleotides and encoded 451 amino acids. The NS2 gene shared 98.89% and 98.89% homology with that of B strain at respectively nucleotide and amino acid level. |
| Keywords: | GPV NS2 gene cloning sequence analysis |
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